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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens.
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Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens.

机译:两种快速培养方法在临床标本中检测巨细胞病毒的比较。

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The conventional virus isolation technique was compared with a 24-h shell vial centrifugation culture technique and with a 48-h tube culture method for the detection of cytomegalovirus (CMV) in MRC-5 cells. Of 200 clinical specimens tested, 41 were positive for CMV by at least one procedure. Indirect immunoperoxidase staining was positive for 32 (78.0%) of 41 specimens in the tube culture method and for 30 (73.2%) of 41 specimens in the shell vial centrifugation method. CMV was detected in 23 (56.1%) of 41 specimens by the development of cytopathic effect within 14 days.
机译:将常规病毒分离技术与24小时壳小瓶离心培养技术和48小时管培养方法进行了比较,以检测MRC-5细胞中的巨细胞病毒(CMV)。在至少200种临床样本中,至少有41种CMV阳性。间接免疫过氧化物酶染色在试管培养方法中对41个样本中的32个(78.0%)呈阳性,在壳管离心法中对41个样本中的30个(73.2%)呈阳性。通过在14天内发展出细胞病变效应,在41个样本中的23个(56.1%)中检测到CMV。

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