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首页> 外文期刊>Journal of Clinical Microbiology >Comparative evaluation of the oxoid signal and Roche Septi-Chek blood culture systems.
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Comparative evaluation of the oxoid signal and Roche Septi-Chek blood culture systems.

机译：oxoid信号和Roche Septi-Chek血液培养系统的比较评估。

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The Oxoid Signal blood culture system (Oxoid USA, Inc., Columbia, Md.) was compared with the Roche Septi-Chek system (Roche Diagnostics, Div. Hoffmann-La Roche Inc., Nutley, N.J.), with the latter consisting of a tryptic soy broth (R-TSB) bottle with an attached agar slide unit and a Columbia broth bottle. A total of 5,034 cultures with equal volumes of blood in each bottle were processed. Overall, more organisms were recovered in the R-TSB bottle than in the Signal bottle, with significantly more aerobic organisms (Pseudomonas spp., Acinetobacter spp., and yeasts) recovered in the R-TSB bottles and anaerobes and viridans group streptococci recovered in Signal bottles. Approximately equivalent numbers of organisms were recovered in the Signal and Columbia broth bottles. The times of detection were essentially identical with the three blood culture broth systems. During the study, 30.6% of the Signal bottles had a positive indicator of growth, of which 1,103 (71.7%) were false-positive cultures. Additionally, nonviable organisms resembling streptococci were observed in 13.7% of the Signal bottles that were Gram stained and in unioculated blood culture bottles. With appropriate modifications of the preparation of the media, the latter problem can be eliminated.
机译：将Oxoid Signal血液培养系统（Oxoid USA，Inc.，Columbia，MD）与Roche Septi-Chek系统（Roche Diagnostics，Div。Hoffmann-La Roche Inc.，Nutley，NJ）进行了比较，后者包括一个胰蛋白酶大豆肉汤（R-TSB）瓶，带有一个琼脂滑动装置和一个哥伦比亚肉汤瓶。每个瓶中总共处理了5,034个血液量相等的培养物。总体而言，在R-TSB瓶中回收的生物比在信号瓶中回收的菌多，在R-TSB瓶中回收的好氧生物（假单胞菌属，不动杆菌属和酵母菌）明显更多，而厌氧菌和绿化菌类链球菌的回收率则高于信号瓶。在Signal和Columbia肉汤瓶中回收了大约等量的生物。检测时间与三种血液培养液系统基本相同。在研究过程中，有30.6％的Signal瓶具有生长的阳性指标，其中1,103（71.7％）是假阳性培养物。此外，在13.7％的革兰氏染色信号瓶和未接种的血液培养瓶中观察到了类似于链球菌的无生命生物。通过适当修改介质的制备，可以消除后一个问题。

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