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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus.
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Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus.

机译：评价直接免疫荧光，酶免疫法，离心培养和常规培养以检测呼吸道合胞病毒。

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摘要

Four methods of detecting respiratory syncytial virus (RSV) from clinical specimens were evaluated. A total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of RSV by direct immunofluorescence assay (DFA), enzyme immunoassay (EIA) (Kallestad Pathfinder), shell vial centrifugation culture (SVC), and conventional culture. DFA identified 146 (83%) of the 175 positive cases, EIA detected 153 (87%), SVC detected 127 (73%), and conventional culture detected 70 (40%). Conventional culture isolated an additional 19 respiratory viruses other than RSV. DFA and EIA were able to detect nonviable virus not isolated by a culture method, and SVC isolated low-titer virus not detected by conventional culture. DFA and EIA gave similar results; however, the EIA system was less dependent on technical expertise. The use of SVC enhanced the conventional culture system with 63 RSV isolates not recovered from the tube culture. We recommend complementary use of both culture and nonculture methods in the detection of RSV.
机译：评估了从临床标本中检测呼吸道合胞病毒（RSV）的四种方法。通过直接免疫荧光测定（DFA），酶免疫测定（EIA）（Kallestad Pathfinder），壳小瓶离心培养（SVC）和常规方法，同时检测了410个由鼻咽洗液，抽吸液和拭子组成的标本中是否存在RSV文化。在175例阳性病例中，DFA检出146例（83％），EIA检出153例（87％），SVC检出127例（73％），常规培养检出70例（40％）。传统培养分离出除RSV之外的其他19种呼吸道病毒。 DFA和EIA能够检测出未通过培养方法分离出的无生命病毒，而SVC则分离出了常规培养未检测到的低滴度病毒。 DFA和EIA得出了类似的结果;但是，EIA系统较少依赖技术专家。 SVC的使用增强了常规培养系统，其中包含63种未从试管培养物中回收的RSV分离株。我们建议在检测RSV时同时使用文化和非文化方法。

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