Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

S N Cho; G M van der Vliet; S Park; S H Baik; S K Kim; Y Chong; A H Kolk; P R Klatser; J D Kim

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Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

机译：比色微孔板杂交检测痰标本中扩增的结核分枝杆菌DNA。

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We developed a colorimetric microwell plate hybridization assay (CoMPHA) for the specific detection of 5'-biotinylated amplified Mycobacterium tuberculosis DNA. The optical densities of the CoMPHA corresponded to the initial amounts of purified template DNA. Here, we show that the CoMPHA is useful in distinguishing the PCR-positive and PCR-negative samples.

机译：我们开发了比色微孔板杂交测定法（CoMPHA），用于5'-生物素化扩增的结核分枝杆菌DNA的特异性检测。 CoMPHA的光密度对应于纯化模板DNA的初始量。在这里，我们表明CoMPHA在区分PCR阳性和PCR阴性样品中很有用。

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《Journal of Clinical Microbiology》 |1995年第3期|共3页
作者
S N Cho; G M van der Vliet; S Park; S H Baik; S K Kim; Y Chong; A H Kolk; P R Klatser; J D Kim;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection [J] . Michelon Candice Tosi, Rosso Franciele, Schmid Karen Barros, Memorias do Instituto Oswaldo Cruz . 2011,第2期

机译：比色微孔板逆杂交检测结核分枝杆菌
2. A PCR-Colorimetric Microwell Plate Hybridization Assay for Detection of Mycobacterium tuberculosis and M. avium from Culture Samples and Ziehl-Neelsen-Positive Smears [J] . Maria Cristina Rossi, Andrea Gori, Gianguglielmo Zehender, Journal of Clinical Microbiology . 2000,第5期

机译：PCR-比色微孔板杂交检测培养样品和Ziehl-Neelsen-阳性涂片中结核分枝杆菌和鸟分枝杆菌
3. Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and Roche PCR-microwell plate hybridization method (AMPLICOR MYCOBACTERIUM) for direct detection of mycobacteria. [J] . S Ichiyama, Y Iinuma, Y Tawada, Journal of Clinical Microbiology . 1996,第1期

机译：直接检测分枝杆菌的Gen-Probe扩增结核分枝杆菌直接试验和Roche PCR-微孔板杂交方法（AMPLICOR MYCOBACTERIUM）的评估。
4. Spectrophotometric vs. colorimetric analysis of Mycobacterium tuberculosis population growth curves in resazurin assay [C] . Eugene B. Postnikov, Anastasia I. Lavrova, Andrey A. Khalin, Russian Foundation for Basic Research;Russian Academy of Sciences;International Symposium on Optics and Biophotonics: Saratov Fall Meeting . 2019

机译：刃天青测定中结核分枝杆菌种群生长曲线的分光光度与比色分析
5. An immunocapture assay for the detection of Mycobacterium avium subsp. paratuberculosis for the diagnosis of Johne's disease. [D] . Chui, Linda Wai-Ling. 2005

机译：一种用于检测鸟分枝杆菌亚种的免疫捕获测定。副结核病诊断约翰氏病。
6. Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples. [O] . S N Cho, G M van der Vliet, S Park, 1995

机译：比色微孔板杂交检测痰标本中扩增的结核分枝杆菌DNA。
7. Colorimetric microwell plate reverse-hybridization assay for Mycobacterium tuberculosis detection [O] . Candice Tosi Michelon, Franciele Rosso, Karen Barros Schmid, 2011

机译：比色微孔板反向杂交法检测结核分枝杆菌

Colorimetric microwell plate hybridization assay for detection of amplified Mycobacterium tuberculosis DNA from sputum samples.

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