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首页> 外文期刊>Journal of Clinical Microbiology >Detection and quantitation of the glucuronoxylomannan-like polysaccharide antigen from clinical and nonclinical isolates of Trichosporon beigelii and implications for pathogenicity.
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Detection and quantitation of the glucuronoxylomannan-like polysaccharide antigen from clinical and nonclinical isolates of Trichosporon beigelii and implications for pathogenicity.

机译：从米色毛癣菌的临床和非临床分离株中检测和定量葡糖醛酸氧甘露聚糖样多糖抗原及其致病性。

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Sera from patients with systemic infections caused by the opportunistic fungus Trichosporon beigelii have been shown to cross-react with anticryptococcal antibodies. We quantitatively compared the amounts of antigen produced and examined the expression of O-acetyl epitopes from 35 strains of T. beigelii isolated from deep and superficial infections. By counterimmunoelectrophoresis, 10 of 10 isolates from deep infections were positive for polysaccharide, compared with 7 of 13 isolates from superficial infections (P = 0.02). All 23 strains tested were positive for polysaccharide when screened by immunodot. By enzyme immunoassay, the cross-reactive antigen produced by deep isolates (n = 9) had a mean titer of 1:5,500. In contrast, superficial isolates (n = 22) produced significantly less antigen than the deep isolates (P < 0.001), with a mean titer of 1:700. Isolates from environmental sources (n = 3) were similar to the superficial isolates, with a mean titer of 1:600. The mean concentrations +/- standard errors of cross-reactive polysaccharide released by deep isolates and superficial isolates were 3.09 +/- 0.44 and 1.74 +/- 0.30 micrograms/ml, respectively, when measured by rocket immunoelectrophoresis (P = 0.02). O-Acetyl epitopes were detected on polysaccharide from 8 of 9 strains of T. beigelii isolated from deep sources, while only 2 of 12 superficial isolates expressed detectable O-acetyl epitopes (P = 0.01). Thus, while all isolates of T. beigelii tested were capable of producing glucuronoxylomannan-like cross-reactive antigen, pathogenic isolates produced significantly more antigen than superficial or environmental isolates. Furthermore, significantly more pathogenic isolates than superficial or environmental isolates expressed antigen that was O acetylated.
机译：由机会性真菌米氏弧菌引起的全身感染患者的血清已显示与抗隐球菌抗体发生交叉反应。我们定量比较了产生的抗原量，并检查了从深部和浅表感染中分离出的35株米色锥虫的O-乙酰基表位的表达。通过反免疫电泳，深部感染的10株分离株中有10株呈阳性，而浅表感染的13株分离株中有7株（P = 0.02）。当通过免疫点筛选时，所有测试的23个菌株的多糖均为阳性。通过酶免疫法，深分离株（n = 9）产生的交叉反应抗原的平均滴度为1：5,500。相比之下，浅层分离株（n = 22）产生的抗原明显少于深层分离株（P <0.001），平均滴度为1：700。来自环境的分离株（n = 3）与表面分离株相似，平均滴度为1：600。当通过火箭免疫电泳法测定时，由深层分离物和浅层分离物释放的交叉反应多糖的平均浓度+/-标准误差分别为3.09 +/- 0.44和1.74 +/- 0.30微克/ ml（P = 0.02）。在从深层来源分离的9种米色锥虫菌株中，有8种在多糖上检测到O-乙酰基表位，而在12种表面分离株中只有2种表达了可检测到的O-乙酰基表位（P = 0.01）。因此，尽管所测试的米色锥虫的所有分离物都能够产生葡糖醛酸氧甘露聚糖样的交叉反应抗原，但是病原性分离物比表面或环境分离物产生的抗原要多得多。此外，比表面或环境分离株表达的抗原被O乙酰化的病原分离株要多得多。

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