Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.

W A Scott; D Brambilla; E Siwak; C Beatty; J Bremer; R W Coombs; H Farzadegan; S A Fiscus; S M Hammer; F B Hollinger; N Khan; S Rasheed; P S Reichelderfer

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Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.

机译：评估用于人类1型免疫缺陷病毒定量微量共培养测定的实时质量控制的传染性标准。艾滋病临床试验小组的参与实验室。

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Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infected cell suspensions and culture supernatants were compared among seven assays sites. There was no significant change in titer during 1 year of storage. The overall standard deviation for infected cell suspensions was approximately 0.8 log10 virus titer. A method for detecting deviant assay results was developed and was used to identify two donor cell preparations (n = 54) that gave consistently low titers.

机译：在7个检测位点之间比较了冷冻保存的1型人免疫缺陷病毒1型感染细胞悬浮液和培养上清液的定量微培养分析。储存1年中的滴度没有明显变化。感染细胞悬液的总体标准偏差约为0.8 log10病毒滴度。开发了一种检测异常测定结果的方法，该方法用于鉴定滴度始终较低的两种供体细胞制剂（n = 54）。

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《Journal of Clinical Microbiology》 |1996年第9期|共4页
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W A Scott; D Brambilla; E Siwak; C Beatty; J Bremer; R W Coombs; H Farzadegan; S A Fiscus; S M Hammer; F B Hollinger; N Khan; S Rasheed; P S Reichelderfer;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Establishment of a quality assurance program for human immunodeficiency virus type 1 DNA polymerase chain reaction assays by the AIDS Clinical Trials Group. ACTG PCR Working Group, and the ACTG PCR Virology Laboratories. [J] . J B Jackson, J Drew, H J Lin, Journal of Clinical Microbiology . 1993,第12期

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2. Standardization of sensitive human immunodeficiency virus coculture procedures and establishment of a multicenter quality assurance program for the AIDS Clinical Trials Group. The NIH/NIAID/DAIDS/ACTG Virology Laboratories. [J] . F B Hollinger, J W Bremer, L E Myers, Journal of Clinical Microbiology . 1992,第7期

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6. Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group. [O] . W A Scott, D Brambilla, E Siwak, 1996

机译：评估用于人类1型免疫缺陷病毒定量微量共培养测定的实时质量控制的传染性标准。艾滋病临床试验小组的参与实验室。
7. Establishment of a quality assurance program for human immunodeficiency virus type 1 DNA polymerase chain reaction assays by the AIDS Clinical Trials Group. ACTG PCR Working Group, and the ACTG PCR Virology Laboratories [O] . J B Jackson, J Drew, H J Lin, 1993

机译：建立人类免疫缺陷病毒的质量保证计划1型艾滋病临床试验组1 DNA聚合酶链反应测定。 Actg PCR工作组和Actg PCR病毒学实验室

Evaluation of an infectivity standard for real-time quality control of human immunodeficiency virus type 1 quantitative micrococulture assays. Participating Laboratories of The AIDS Clinical Trials Group.

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