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首页> 外文期刊>Journal of Clinical Microbiology >Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing
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Inhibition of PCR in Genital and Urine Specimens Submitted for Chlamydia trachomatis Testing

机译：提交沙眼衣原体检测的生殖器和尿液样本中PCR的抑制作用

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We determined the frequency of PCR inhibition in genital and urine specimens submitted for Chlamydia trachomatis testing using the internal control DNA provided with the COBAS AMPLICOR C. trachomatis test and assessed methods to remove it. Inhibition occurred in 65 of 906 (7%) cervical swabs, 23 of 51 (45%) urethral swabs, and 2 of 175 (1.1%) urine samples. Overall, inhibition was eliminated in processed specimens after storage at 4°C in 77 of 90 specimens (86%), freezing at ?70°C in 59 of 82 specimens (72%), storage at 4°C followed by either 1:100 dilution in 37 of 43 specimens (86%) or 1:10 dilution in 42 of 47 specimens (89%), and phenol-chloroform extraction in 79 of 80 specimens (99%). No positive specimens were missed due to inhibition. We conclude that PCR inhibition is rare with urine specimens and infrequent with endocervical swabs but occurs frequently with urethral swabs. The frequency of PCR inhibition may be significantly reduced by methods which can be easily incorporated into the processing of specimens.
机译：我们使用COBAS AMPLICOR C随附的内部对照DNA确定了提交沙眼衣原体检测的生殖器官和尿液样本中PCR抑制的频率。沙眼测试并评估了去除沙眼的方法。 906份（7％）宫颈拭子中有65份，51份（45％）尿道拭子中有23份和175份（1.1％）尿液样本中有2份发生抑制作用。总的来说，在90°C的样品中有77个（86％）在4°C下保存后，在82°C的样品中有59个在72°C下冻结（在70°C下冷冻），然后在4°C下保存：在43个样本中的37个（86％）中稀释100倍，在47个样本中的42个（89％）中以1:10稀释，在80个样本中的79个（99％）中进行苯酚-氯仿萃取。没有因抑制而遗漏的阳性标本。我们得出的结论是，PCR抑制作用在尿液标本中很少见，在宫颈内拭子中很少见，而在尿道拭子中则很常见。 PCR抑制的频率可以通过可轻松纳入标本处理的方法显着降低。

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