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首页> 外文期刊>Journal of Clinical Microbiology >Immunological procedure for the rapid and specific identification of Coccidioides immitis cultures.
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Immunological procedure for the rapid and specific identification of Coccidioides immitis cultures.

机译：快速，特异性鉴定球虫科球菌培养物的免疫程序。

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An immunological procedure for the rapid and specific identification of Coccidioides immitis isolates has been developed. The specificity of the procedure is based on the fact that C. immitis produces antigens that are not produced by morphologically similar fungi. The procedure involved the transfer of heavy mold-form inocula to flasks that contained small volumes of brain heart infusion broth. Shake cultures were grown at 25 degrees C on a gyratory shaker at 150 rpm. The concentrated supernatant antigens so obtained from broth cultures were tested in parallel with coccidioidin by a microimmunodiffusion technique against C. immitis antiserum. The ability of the immunological procedure to identify C. immitis cultures was evaluated by testing the supernatant antigens of 166 unknown isolates, many of which, by gross or microscopic examination or both, resembled C. immitis or belonged to the family Gymnoascaceae. Each culture was also identified by conventional laboratory procedures. Comparative evaluation showed that the immunological test for C. immitis was 100% sensitive and specific. The diagnostic precipitin bands that were produced by the interaction of the C. immitis supernatants and antisera were shown to consist of (i) a heat-stable antigen comparable to that active in the tube precipitin test and (ii) two heat-labile antigens, one of which was associated with complement fixation reactivity. With pure cultures, this immunological procedure permitted the identification of C. immitis isolates within 5 days.
机译：已经开发出一种免疫学方法，用于快速，特异性地鉴定球孢子菌的炎性分离物。该方法的特异性是基于以下事实：C。immitis产生的抗原不是形态相似的真菌产生的。该程序涉及将重的霉菌形式的接种物转移到装有少量脑心浸液的培养瓶中。摇动培养物在25摄氏度的旋转摇床上以150 rpm的速度生长。通过微免疫扩散技术针对C. immitis抗血清，从肉汤培养物中获得的浓缩的上清液抗原与球孢菌素平行进行测试。通过测试166种未知分离物的上清抗原来评估免疫学程序鉴定丙种梭菌培养物的能力，其中许多分离物通过肉眼或显微镜检查或两者均类似于丙种梭菌或属于裸子草科。每种培养物也通过常规实验室程序鉴定。对比评估表明，对秀丽隐杆线虫的免疫学测试是100％灵敏和特异的。由肠炎衣原体上清液和抗血清的相互作用产生的诊断性沉淀素条带显示由（i）与管沉淀素测试中的活性相当的热稳定抗原和（ii）两种对热不稳定的抗原组成，其中之一与补体固定反应性有关。对于纯培养物，这种免疫学方法允许在5天内鉴定出C.炎性肠炎分离株。

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