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首页> 外文期刊>Journal of Clinical Microbiology >Analysis of parameters affecting the hemagglutination activity of Escherichia coli possessing colonization factor antigens: improved medium for observing erythrocyte agglutination.
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Analysis of parameters affecting the hemagglutination activity of Escherichia coli possessing colonization factor antigens: improved medium for observing erythrocyte agglutination.

机译：影响具有定居因子抗原的大肠杆菌的血凝活性的参数分析：用于观察红细胞凝集的改良培养基。

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摘要

The hemagglutination (HA) activity of two strains of Escherichia coli, each possessing different colonization factor antigens (CFA), was examined under different test conditions. The effects of ionic strength, temperature, pH, cations, and reaction surface on erythrocyte (RBC) agglutination were analyzed. Strain H-10407 (CFA/I) caused the agglutination of human, bovine, and chicken RBC, whereas strain CL-9699 (CFA/II) agglutinated only bovine and chicken RBC. The HA activity of both strains increased with decreasing ionic strength, pH, and temperature, the effects of temperature being negligible at low ionic strength. When accounting for ionic strength, the presence of Ca2+, Mg2+, Fe2+, or Fe3+ ions did not increase the HA activity of these bacteria. Optimum conditions for HA of reactive RBC by bacteria included low ionic strength (less than 50 mM) and slightly acidic pH (6.0 to 7.0). Use of a low-ionic-strength medium permitted application of microtitration methods to visualize the HA reactions. Storage of RBC in low-ionic-strength medium did not change their HA properties, and the use of this medium proved superior to saline in overcoming HA variation observed with different preparations of RBC.
机译：在不同的测试条件下检查了两株各自具有不同定殖因子抗原（CFA）的大肠杆菌的血凝（HA）活性。分析了离子强度，温度，pH，阳离子和反应表面对红细胞（RBC）凝集的影响。菌株H-10407（CFA / I）引起人，牛和鸡RBC的凝集，而菌株CL-9699（CFA / II）仅凝集牛和鸡RBC。两种菌株的HA活性都随着离子强度，pH和温度的降低而增加，在低离子强度下温度的影响可忽略不计。考虑离子强度时，Ca2 +，Mg2 +，Fe2 +或Fe3 +离子的存在不会增加这些细菌的HA活性。细菌对反应性红细胞进行HA的最佳条件包括低离子强度（小于50 mM）和弱酸性pH（6.0至7.0）。使用低离子强度的介质可以应用微量滴定法来可视化HA反应。将RBC储存在低离子强度的培养基中不会改变其HA特性，并且在克服不同RBC制剂观察到的HA变化方面，使用该培养基证明优于盐水。

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