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首页> 外文期刊>Journal of Clinical Microbiology >New York City medium for enhanced recovery of Mycoplasma pneumoniae from clinical specimens.
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New York City medium for enhanced recovery of Mycoplasma pneumoniae from clinical specimens.

机译：纽约市用于增强从临床标本中回收肺炎支原体的培养基。

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Modified New York City (MNYC) medium and PPLO medium without methylene blue (PPLO agar) were compared for their ability to support the growth of Mycoplasma pneumoniae from clinical specimens. Pharyngeal specimens were collected from 1,070 college students who visited the Syracuse University Student Health Center. Of these patients, 623 were symptomatic for respiratory infection, and the remaining 447 were asymptomatic for respiratory illness. Throat swabs were inoculated into PPLO broths, and these broths were subcultured onto MNYC medium and PPLO agar after 3 and 14 days of incubation. A total of 222 (20.7%) clinical isolates of M. pneumoniae were recovered on these solid media, with the majority of the isolates (196) recovered from symptomatic patients. All isolates grew on MNYC medium, whereas five isolates failed to grow on PPLO agar. All isolates of M. pneumoniae recovered from symptomatic patients were detected on MNYC medium within 1 to 5 days of incubation, whereas 5 to 7 days of incubation were required before mycoplasmal growth was detected on PPLO agar. Over 86% of these mycoplasma isolates were detected on MNYC medium within 3 days of incubation and before the detection of any mycoplasmal growth on PPLO agar. A similar pattern of recovery times was observed for mycoplasmas isolated from asymptomatic patients. The results of this study have shown that MNYC medium is better than PPLO agar in supporting the rapid growth of M. pneumoniae from clinical specimens after 72-h blind subculture in PPLO glucose broth.
机译：比较了改良的纽约市（MNYC）培养基和不含亚甲蓝的PPLO培养基（PPLO琼脂），它们从临床标本中支持肺炎支原体生长的能力。从访问锡拉丘兹大学学生健康中心的1,070名大学生中收集咽标本。在这些患者中，有623例因呼吸道感染而有症状，其余447例因呼吸道疾病而无症状。将喉拭子接种到PPLO肉汤中，孵育3天和14天后，将这些肉汤传代培养到MNYC培养基和PPLO琼脂上。在这些固体培养基上共回收了222株（20.7％）肺炎支原体临床分离株，其中大多数分离株（196）从有症状的患者中回收。所有分离株均在MNYC培养基上生长，而5个分离株未能在PPLO琼脂上生长。从症状患者中回收的所有肺炎支原体分离株均在孵育后1到5天内在MNYC培养基上检测到，而在PPLO琼脂上检测到支原体生长之前需要孵育5到7天。在孵育的三天内以及在检测到PPLO琼脂上的任何支原体生长之前，在MNYC培养基上检测到超过86％的这些支原体分离株。从无症状患者中分离出的支原体观察到相似的恢复时间模式。这项研究的结果表明，MNYC培养基比PPLO琼脂更好地支持了在PPLO葡萄糖肉汤中进行72 h盲传代培养后临床标本中肺炎支原体的快速生长。

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