4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan.

J R Davis; C E Stager; G F Araj

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4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan.

机译：用9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶4-h鉴定铜绿假单胞菌。

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A total of 361 gram-negative bacilli were evaluated for their ability to grow in the presence of 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan. The minimum time required for the production of visual turbidity in brain heart infusion broth was determined to be 4 h in shake cultures at 35 degrees C. The minimal inhibitory concentration (MIC) of 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan in brain heart infusion broth was determined for 174 isolates. The MICs for all 40 Pseudomonas aeruginosa isolates tested were greater than 50 micrograms/ml. The MICs for the other 53 pseudomonads tested were less than or equal to 5 micrograms/ml. Among 81 other gram-negative bacilli tested, the MICs for 4 were 15 micrograms/ml, the MICs for 15 were 10 micrograms/ml, the MICs for 21 were 5 micrograms/ml, and the MICs for 41 were less than or equal to 1 micrograms/ml. Based on these data, 361 gram-negative bacilli were inoculated into brain heart infusion broth containing 15 micrograms of 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan per ml and incubated on a shaker for 4 h. The only bacteria that produced visual turbidity were identified as P. aeruginosa (170 of 170 isolates).

机译：评估总共361克阴性杆菌在9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶存在下的生长能力。在35摄氏度的摇动培养中，确定脑心输液肉汤中产生视觉浊度所需的最短时间为4小时。9-氯-9-（4-二乙基氨基苯基）-10的最小抑菌浓度（MIC）测定了174株分离物在脑心浸液中的β-苯ac啶。测试的所有40个铜绿假单胞菌分离物的MIC均大于50微克/毫升。其他53个假单核细胞的MIC小于或等于5微克/毫升。在其他81种革兰氏阴性杆菌中，MIC的4 MIC为15微克/毫升，MIC的15 MIC为10微克/毫升，MIC的21 MIC为5微克/毫升，41的MIC小于或等于1微克/毫升。根据这些数据，将361克阴性杆菌接种到每毫升含15微克9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶的脑心浸液中，并在振荡器上孵育4小时。唯一产生视觉混浊的细菌被鉴定为铜绿假单胞菌（170个分离株中的170个）。

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《Journal of Clinical Microbiology》 |1983年第6期|共3页
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J R Davis; C E Stager; G F Araj;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Use of 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan as a primary medium for recovery of Pseudomonas aeruginosa from clinical specimens. [J] . G F Araj Journal of Clinical Microbiology . 1984,第3期

机译：使用9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶作为主要培养基从临床标本中回收铜绿假单胞菌。
2. Selective medium for Pseudomonas cepacia containing 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan and polymyxin B sulfate. [J] . B J Wu, S T Thompson Applied and Environmental Microbiology . 1984,第4期

机译：洋葱假单胞菌的选择性培养基，其中含有9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶和多粘菌素B硫酸盐。
3. Identification of Pseudomonas aeruginosa by using a disk of phenanthroline and 9-chloro-9-[4-(diethylamino)phenyl]-9,10-dihydro-10-phenylacridine hydrochloride and by cell agglutination testing with monoclonal antibodies. [J] . S Fujita, A Tonohata, T Matsuoka, Journal of Clinical Microbiology . 1992,第10期

机译：通过使用菲咯啉和9-氯-9- [4-（二乙基氨基）苯基] -9,10-二氢-10-苯基ac啶盐酸盐的圆盘并通过单克隆抗体的细胞凝集试验鉴定铜绿假单胞菌。
4. Identification of Genes Encoding Receptors for Six Pseudomonas aeruginosa Phages [C] . Jiajia You, Xiaoli Cui, Li Sun, International conference on applied biotechnology . 2018

机译：鉴定铜绿假单胞菌六个噬菌体受体的基因
5. Identification of Broad Host Range Phage that Antagonize Multidrug Resistant Pseudomonas Aeruginosa and Their Therapeutic Potential to Restore Antibiotic Susceptibility Among These Pathogens [D] . Lake, Alexandra Elizabeth. 2020

机译：鉴定抗拮抗多药抗性假单胞菌铜绿假单胞菌的广泛宿主范围噬菌体及其治疗潜力在这些病原体中恢复抗生素敏感性
6. 4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan. [O] . J R Davis, C E Stager, G F Araj 1983

机译：用9-氯-9-（4-二乙基氨基苯基）-10-苯基ac啶酮进行4-h铜绿假单胞菌鉴定。
7. Cloning of a catabolite repression control (crc) gene from Pseudomonas aeruginosa, expression of the gene in Escherichia coli, and identification of the gene product in Pseudomonas aeruginosa. [O] . MacGregor, C H, Wolff, J A, Arora, S K, 1991

机译：铜绿假单胞菌的分解代谢物阻遏控制（crc）基因的克隆，该基因在大肠杆菌中的表达以及铜绿假单胞菌的基因产物的鉴定。

4-h Identification of Pseudomonas aeruginosa with 9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan.

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