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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California.
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Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California.

机译:从加利福尼亚州的凤头虱分离的伯氏疏螺旋体菌株的表征。

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In a survey of 1,714 adult Ixodes pacificus ticks collected in northern California, 24 (1.4%) were found to be infected with spirochetes that reacted with an anti-Borrelia burgdorferi polyvalent conjugate in direct immunofluorescence tests. Eleven isolates of B. burgdorferi from these ticks were characterized by monoclonal antibody, polyacrylamide gel electrophoresis, and Western blot (immunoblot) analyses. Ten of the isolates had molecular and antigenic characteristics similar to those of other U.S. isolates. One strain, cloned by limiting-dilution techniques, was different from any previously reported U.S. strain, but similar to reported European strains. The cloned strain, DN127-Cl9-2, did not react with monoclonal antibodies to Osp A and Osp B major proteins found in most of the U.S. strains. It exhibited an abundant protein with an apparent molecular weight of 25,000.
机译:在对加利福尼亚北部收集的1,714个成年的美洲x的调查中,有24个(1.4%)被螺旋体感染,该螺旋体在直接免疫荧光测试中与抗伯氏疏螺旋体多价缀合物反应。通过单克隆抗体,聚丙烯酰胺凝胶电泳和Western印迹(免疫印迹)分析对11种来自这些壁虱的B. burgdorferi菌株进行了鉴定。十个分离株具有与其他美国分离株相似的分子和抗原特性。通过有限稀释技术克隆的一种菌株不同于任何先前报道的美国菌株,但是与报道的欧洲菌株相似。克隆的菌株DN127-Cl9-2不与大多数美国菌株中发现的针对Osp A和Osp B主要蛋白的单克隆抗体反应。它显示出丰富的蛋白质,表观分子量为25,000。

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