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首页> 外文期刊>Journal of Clinical Microbiology >Production and characterization of yeast killer toxin monoclonal antibodies.
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Production and characterization of yeast killer toxin monoclonal antibodies.

机译：酵母杀伤毒素单克隆抗体的生产和表征。

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Monoclonal antibodies were obtained after fusion of mouse myeloma cells with spleen cells isolated from mice primed with a crude extract of yeast killer toxin produced by a strain of Hansenula anomala. Hybridomas were selected by specific immunoassay reaction of their fluid with crude yeast killer toxin extract. Among the monoclonal antibodies, which were characterized by the Western blot technique, one (designated KT4) proved to have precipitating properties, thus permitting the neutralization of the killer activity of the toxin. Experiments in double immunodiffusion showed that monoclonal antibody KT4 produced homologous precipitin bands by reacting with either the crude toxin used as immunogen or a toxic extract of Hansenula mrakii. It is suggested that these monoclonal antibodies will be useful for the purification, characterization, and understanding of the bioactions of yeast killer toxins.
机译：在小鼠骨髓瘤细胞与从小鼠中分离的脾细胞融合后获得单克隆抗体，所述小鼠用汉逊酵母异常菌株产生的酵母杀伤毒素的粗提取物引发。通过杂交瘤细胞的液体与粗酵母杀手毒素提取物的特异性免疫分析反应来选择杂交瘤。在通过Western印迹技术表征的单克隆抗体中，一种（称为KT4）被证明具有沉淀特性，因此可以中和毒素的杀伤活性。双重免疫扩散实验表明，单克隆抗体KT4通过与用作免疫原的粗毒素或汉氏汉逊酵母的有毒提取物反应，产生同源的沉淀蛋白条带。提示这些单克隆抗体可用于纯化，表征和理解酵母杀手毒素的生物作用。

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