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首页> 外文期刊>Journal of Clinical Microbiology >Identification of coagulase-negative staphylococci with the API STAPH-IDENT system.
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Identification of coagulase-negative staphylococci with the API STAPH-IDENT system.

机译:用API STAPH-IDENT系统鉴定凝固酶阴性葡萄球菌。

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A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a miniaturized biochemical test strip that incorporates four synthetic chromogenic substrates, urea, arginine, and four carbohydrates and that requires only a 5-h test period. Use of the STAPH-IDENT system alone allowed correct or partly correct classification of 67% (201 of 300) of the study isolates. However, if a supplemental test was performed (most often novobiocin susceptibility), correct classification of an additional 25.7% (77) was possible, for a total of 92.7% of isolates identified to the species level. Species correctly identified included 94% (116 of 123) of Staphylococcus epidermidis isolates, 98% (63 of 64) of S. saprophyticus, 71% (34 of 48) of S. hominis, 100% (22) of S. simulans, 100% (18) of S. haemolyticus, 100% (17) of S. warneri, and 100% (8) of S. capitis. Fourteen percent (42 of 300) of profile codes encountered in this study were not included in the STAPH-IDENT profile register, but were included in Analytab Products' expanded computer data base.
机译:与API STAPH-IDENT系统(Analytab产品)并行测试了300个临床衍生的凝固酶阴性葡萄球菌分离株,并在Kloos和Schleifer简化的鉴定人类葡萄球菌物种的简化方案中进行了14种常规生化测试。 STAPH-IDENT是一种微型生化测试条,它包含四种合成的生色底物,尿素,精氨酸和四种碳水化合物,并且仅需5小时的测试时间。单独使用STAPH-IDENT系统可以对研究分离株的67%(300个中的201个)进行正确或部分正确的分类。但是,如果进行补充测试(大多数情况下是新霉素易感性),则有可能对25.7%的正确分类进行正确分类(77),这样就可以在物种水平上鉴定出92.7%的分离株。正确鉴定出的物种包括表皮葡萄球菌分离株的94%(123个中的116个),腐生链球菌98%(64个中的63个),人型链球菌71%(48个中的34个),simulans的100%(22个), 100%(18)的溶血链球菌,100%(17)的华氏链球菌和100%(8)的头孢葡萄球菌。这项研究中遇到的配置文件代码的百分之十四(300个中的42个)未包含在STAPH-IDENT配置文件寄存器中,而是包含在Analytab产品的扩展计算机数据库中。

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