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首页> 外文期刊>Journal of Clinical Microbiology >Possible common biological and immunological properties for detecting encapsulated strains of Staphylococcus epidermidis.
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Possible common biological and immunological properties for detecting encapsulated strains of Staphylococcus epidermidis.

机译:用于检测表皮葡萄球菌包囊菌株的可能的通用生物学和免疫学性质。

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Twenty strains of capsular type II Staphylococcus epidermidis, determined by the method of Ichiman, were obtained from clinical specimens. Among them, 5, 5, and 10 strains were 4+, 3+, and 2+ in the intensities of their reactions against fluorescent antibody, respectively. Strains exhibiting 4+ and 3+ intensities were mouse virulent and phage nontypable, while 2+ strains were mouse avirulent and phage typable. When three strains randomly selected from each of the mouse-virulent and mouse-avirulent strains were compared in terms of their cell volume indices, all mouse-virulent strains had significantly higher indices (average, 1.86 times) than the mouse-avirulent strains. With intraperitoneal injection of the strains into mice, strains with higher cell volume indices resisted ingestion by peritoneal cells, while strains with low cell volume indices were sensitive to phagocytosis. When the capacity to absorb a definite amount of passive protective activity in rabbit antiserum prepared with capsular type II strains was compared among these strains, 10 to 20 mg of mouse-virulent strains was capable of completely absorbing the passive protective activity, whereas more than 80 mg of the cells was required for similar absorption by mouse-avirulent strains. In ultra-thin sections of three mouse-virulent strains stained with ferritin-conjugated rabbit antiserum, well-defined capsules were detected around cell walls; however, no capsule was seen around the walls of three mouse-avirulent strains.
机译:通过Ichiman方法测定的二十种II型荚膜荚膜葡萄球菌菌株从临床标本中获得。其中,5、5和10株抗荧光抗体的反应强度分别为4 +,3 +和2+。表现出4+和3+强度的菌株对小鼠具有毒力,不能分噬噬菌体,而2+菌株对小鼠无毒,可分噬菌体。当从每种小鼠致病力和小鼠致病力菌株中随机选择三种菌株的细胞体积指数进行比较时,所有小鼠致病性菌株的指数均显着高于小鼠致病力菌株(平均1.86倍)。通过向小鼠腹膜内注射菌株,具有更高细胞体积指数的菌株抵抗了腹膜细胞的摄取,而具有低细胞体积指数的菌株对吞噬作用敏感。在这些菌株中比较了用II型荚膜荚膜菌株制备的兔抗血清吸收一定量的被动保护活性的能力时,有10到20 mg的小鼠毒性菌株能够完全吸收被动保护活性,而超过80种小鼠无毒力的毒株需要相似的细胞吸收量才能达到1 mg。在用铁蛋白缀合的兔抗血清染色的三种小鼠毒力菌株的超薄切片中,在细胞壁周围检测到了明确定义的胶囊。但是,在三种小鼠毒力强毒株的壁上均未见胶囊。

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