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首页> 外文期刊>Journal of Clinical Microbiology >Direct testing of blood culture for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus.
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Direct testing of blood culture for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus.

机译：直接检测血液培养物以检测金黄色葡萄球菌的血清型5和8荚膜多糖。

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摘要

Monoclonal antibodies (MAbs) reactive with serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus have been used to test, by enzyme-linked immunosorbent assay (ELISA), blood culture fluids for the presence of S. aureus. A total of 748 blood cultures from 665 patients yielding 706 bacterial isolates belonging to more than 26 bacterial species were studied. All blood cultures containing bacterial strains belonging to species other than S. aureus were negative in ELISA. All 23 blood cultures containing serotype 5 S. aureus were positive in ELISA with the corresponding MAb. Out of 20 blood cultures containing serotype 8 S. aureus, 19 were positive with the corresponding MAb. All 5 blood cultures containing nontypeable S. aureus were negative in ELISA with both MAbs. This method provides reliable identification of serotype 5 or serotype 8 S. aureus by direct testing of blood culture fluids with ELISA.
机译：与金黄色葡萄球菌的血清型5和8荚膜多糖反应的单克隆抗体（MAb）已用于通过酶联免疫吸附测定（ELISA）测试血液培养液中是否存在金黄色葡萄球菌。研究了来自665名患者的748种血液培养物，产生了706种细菌分离株，这些分离株属于26种以上细菌。 ELISA中所有含有属于金黄色葡萄球菌以外物种的细菌菌株的血液培养物均为阴性。含有5型金黄色葡萄球菌血清型的所有23种血液培养物在ELISA中和相应的MAb均为阳性。在含有8型金黄色葡萄球菌血清型的20种血液培养物中，有19种对于相应的单克隆抗体呈阳性。两种单克隆抗体的ELISA中所有5种含有不可分型金黄色葡萄球菌的血液培养物均为阴性。通过使用ELISA直接检测血液培养液，该方法可可靠地鉴定5型或8型金黄色葡萄球菌。

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