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首页> 外文期刊>Journal of Clinical Microbiology >Pseudomonas aeruginosa flagellar antibodies in patients with cystic fibrosis.
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Pseudomonas aeruginosa flagellar antibodies in patients with cystic fibrosis.

机译：囊性纤维化患者中的铜绿假单胞菌鞭毛抗体。

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An enzyme-linked immunosorbent assay specific for flagellum type (a or b) of Pseudomonas aeruginosa was used to detect serum immunoglobulin antibodies in 98 random outpatients and 14 colonized cystic fibrosis patients. Antibodies were detected to both types of flagella in addition to M-2 lipopolysaccharide. Titers to both flagellar antigens (FlAg) were 10 to 100 times higher in cystic fibrosis patients than in random outpatients of a comparable age group. Mean antibody titers against b-type FlAg were 454 for outpatients (ages newborn to 21 years), whereas the mean titer for cystic fibrosis patients (ages 6 to 21 years) was 51,520. Titers against a-type FlAg were generally lower, with mean outpatient titers of 68 and mean cystic fibrosis patient titers of 34,323. Differences were also seen in antibody titer against M-2 lipopolysaccharide, but these differences did not correspond to M-2 FlAg titers. In 98 random outpatients (ages newborn to 86 years), FlAg titers generally increased with age. To demonstrate further specificity of the enzyme-linked immunosorbent assay for flagellum antibody, Western blots were performed with selected high-titer cystic fibrosis patient sera. Sera that had a high titer (greater than 25,600) for b- or a-type FlAg showed a corresponding reactive band. These results demonstrate that flagellum antibodies are produced in humans in response to P. aeruginosa infection.
机译：一项针对铜绿假单胞菌鞭毛类型（a或b）的酶联免疫吸附法用于检测98位随机门诊患者和14例定植的囊性纤维化患者的血清免疫球蛋白抗体。除M-2脂多糖外，还检测到针对两种鞭毛的抗体。囊性纤维化患者的两种鞭毛抗原（FlAg）的滴度比同年龄组的随机门诊患者的滴度高10至100倍。门诊患者（新生儿至21岁）针对b型FlAg的平均抗体滴度为454，而囊性纤维化患者（6至21岁）的平均抗体滴度为51,520。抗a型FlAg的滴度通常较低，门诊平均滴度为68，囊性纤维化病人平均滴度为34,323。在针对M-2脂多糖的抗体效价上也观察到差异，但是这些差异不对应于M-2 FlAg效价。在98位随机门诊患者（新生儿至86岁）中，FlAg滴度通常随年龄而增加。为了证明鞭毛抗体的酶联免疫吸附试验的进一步特异性，对选定的高滴度囊性纤维化患者血清进行了蛋白质印迹分析。对于b型或a型FlAg具有高滴度（大于25,600）的血清显示相应的反应带。这些结果表明，在人中产生了鞭毛抗体，以响应铜绿假单胞菌感染。

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