Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.

K Midthun; J Valdesuso; A Z Kapikian; Y Hoshino; K Y Green

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Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.

机译：通过单克隆抗体的酶联免疫吸附法鉴定血清型9人轮状病毒。

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Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies.

机译：制备产生针对VP7的单克隆抗体的杂交瘤，VP7是血清型9轮状病毒的主要中和蛋白（菌株W161）。已显示一种单克隆抗体W161-6A1仅中和血清型9轮状病毒株，并在酶联免疫吸附试验中与血清型9轮状病毒特异性反应。用于检测血清型9轮状病毒的免疫测定方法的开发应有助于流行病学研究。

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《Journal of Clinical Microbiology》 |1989年第9期|共3页
作者
K Midthun; J Valdesuso; A Z Kapikian; Y Hoshino; K Y Green;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Comparative sensitivities of solid-phase immune electron microscopy and enzyme-linked immunosorbent assay for serotyping of human rotavirus strains with neutralizing monoclonal antibodies. [J] . G Gerna, A Sarasini, B S Coulson, Journal of Clinical Microbiology . 1988,第7期

机译：固相免疫电子显微镜和酶联免疫吸附测定法对人轮状病毒株中和性单克隆抗体血清分型的比较敏感性。
2. Enzyme-linked immunosorbent assays adapted for serotyping of human rotavirus strains. [J] . G Zissis, J P Lambert Journal of Clinical Microbiology . 1980,第1期

机译：酶联免疫吸附测定适用于人轮状病毒株的血清分型。
3. Detection of human group C rotaviruses by an enzyme-linked immunosorbent assay using monoclonal antibodies. [J] . R Fujii, M Kuzuya, M Hamano, Journal of Clinical Microbiology . 1992,第5期

机译：使用单克隆抗体通过酶联免疫吸附测定检测人组C旋转血管。
4. Development of Monoclonal Antibody-based Enzyme-linked Immunosorbent Assay for Detecting Tetrodotoxin [C] . ZHONG Qing Ping, LIU Yan Ting, SUN Yuan Ming International Conference on Intelligent System and Applied Material . 2012

机译：用于检测四抗毒素的单克隆抗体基酶联免疫吸附测定的研制
5. Development of a monoclonal antibody-based antigen detection enzyme-linked immunosorbent assay (ELISA) for the diagnosis of human toxoplasmosis. [D] . Grushka, Daniel. 2001

机译：基于单克隆抗体的抗原检测酶联免疫吸附测定（ELISA）的开发，用于诊断人类弓形虫病。
6. Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies. [O] . K Midthun, J Valdesuso, A Z Kapikian, 1989

机译：用单克隆抗体通过酶联免疫吸附法鉴定血清型9人轮状病毒。
7. Validity of an Enzyme-Linked Immunosorbent Assay with Serotype-Specific Monoclonal Antibodies for Serotyping Human Rotavirus in Stool Specimens [O] . Shozo Urasawa, Tomoko Urasawa, Koki Taniguchi, 1988

机译：用血清型单克隆抗体进行酶联免疫吸附测定的有效性在粪便标本中血清型人轮状病毒
8. Enzyme-Linked Immunosorbent Assays for Kinins Using High-Affinity Monoclonal Kinin Antibodies. [R] . Odya, C. E., Lee, C. H. 1990

机译：使用高亲和力单克隆Kinin抗体进行酶联免疫吸附试验。

Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies.

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