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首页> 外文期刊>Journal of Clinical Microbiology >Effect of delay in processing on lysis-centrifugation blood culture results from marrow transplant patients.
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Effect of delay in processing on lysis-centrifugation blood culture results from marrow transplant patients.

机译：延迟处理对骨髓移植患者裂解离心血液培养结果的影响。

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The effect of delay in processing on results of lysis-centrifugation (LC; Isolator) blood cultures was assessed in 4,577 paired blood specimens. Blood specimens were obtained at all hours from 384 febrile marrow transplant patients with indwelling venous catheters and were processed by the LC technique and by a conventional two-bottle method. Most patients (84%) were receiving broad-spectrum antibiotics at the time of blood culture. Specimens were delivered to the laboratory, where Isolator tubes were held at 35 degrees C and processed in batches between 0700 and 1730 h daily. This procedure resulted in a delay beyond the manufacturer-suggested processing time of less than 8 h for 1,853 (42%) of the LC cultures. There was no overall difference in the recovery of organisms present in LC cultures processed after being held for 8 to 24 h compared with the conventional two-bottle method. LC methodology had shorter time to detection than the conventional method for detection of Candida spp. and Pseudomonas spp. (P less than 0.05). However, time to detection for Streptococcus spp. and members of the family Enterobacteriaceae, responsible for 16.3% of total isolates, was prolonged significantly by delay in processing when compared with the conventional two-bottle method (P less than 0.01). Results of this study support the recommendation of the manufacturer for processing of Isolator tubes within 8 h or less. Although one can safely delay processing beyond 8 h in terms of total recovery of organisms, such delays were associated with longer time to detection for certain important potentially pathogenic organisms which accounted for a sizeable proportion of blood culture isolates from marrow transplant patients.
机译：在4577对配对的血液样本中评估了处理延迟对裂解离心（LC; Isolator）血液培养结果的影响。在384小时内，从384名有留置静脉导管的高热骨髓移植患者中获取血液样本，并通过LC技术和常规的两瓶法对其进行处理。大多数患者（84％）在血液培养时正在接受广谱抗生素。样品被送到实验室，隔离管被保持在35摄氏度，每天在0700到1730 h之间分批处理。此过程导致超过制造商建议的1853个（42％）LC培养液处理时间少于8小时。与传统的两瓶法相比，在保存8至24小时后处理的LC培养物中存在的生物的回收率没有总体差异。 LC方法的检测时间比常规检测假丝酵母的方法要短。和假单胞菌属。 （P小于0.05）。但是，检测链球菌的时间到了。与传统的两瓶法相比，肠杆菌科的成员（占总分离株的16.3％）因处理延迟而显着延长（P小于0.01）。这项研究的结果支持制造商的建议，要求在8小时或更短的时间内处理隔离管。尽管就生物体的总恢复而言，可以安全地将处理过程延迟到8小时以上，但这种延迟与更长的时间来检测某些重要的潜在致病性生物体有关，这些重要的生物体占了来自骨髓移植患者的大量血液分离株的比例。

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