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首页> 外文期刊>Journal of Clinical Microbiology >Use of modified norleucine-tyrosine broth in identification of Peptostreptococcus anaerobius.
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Use of modified norleucine-tyrosine broth in identification of Peptostreptococcus anaerobius.

机译:修饰的正亮氨酸-酪氨酸肉汤在鉴定厌氧消化链球菌中的用途。

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Gas-liquid chromatography was employed to analyze the volatile and nonvolatile acids produced in modified norleucine-tyrosine (MNT) broth by various gram-positive cocci. The MNT broth consists of 0.5% Trypticase (BBL Microbiology Systems, Cockeysville, Md.), 0.5% yeast extract (Difco Laboratories, Detroit, Mich.), 0.2% L-norleucine, and 0.1% L-tyrosine. The microorganisms included reference strains and clinical isolates of Peptostreptococcus spp. (P. anaerobius, P. asaccharolyticus, P. indolicus, P. magnus, and P. prevotii), Staphylococcus spp. (S. aureus, S. epidermidis, and S. saccharolyticus), and Streptococcus spp. (S. agalactiae, S. intermedius, S. mutans, S. sanguis I, and S. sanguis II). Only Peptostreptococcus anaerobius strains produced caproic and valeric acids in MNT broth cultures. All 11 P. anaerobius strains produced valeric acid in MNT broth, and only 1 strain failed to produce caproic acid in the medium. This unique feature aids in rapid, reliable identification of P. anaerobius with a minimum number of tests.
机译:气相色谱法分析了各种革兰氏阳性球菌在修饰的正亮氨酸-酪氨酸(MNT)肉汤中产生的挥发性和非挥发性酸。 MNT肉汤由0.5%胰蛋白酶(BBL Microbiology Systems,Cockeysville,马里兰州),0.5%酵母提取物(Difco Laboratories,底特律,密歇根州),0.2%L-正亮氨酸和0.1%L-酪氨酸组成。微生物包括参考菌株和Peptostreptococcus spp的临床分离株。 (P. anaerobius,P。asaccharolyticus,P。indolicus,P。magnus和P. prevotii),葡萄球菌属。 (金黄色葡萄球菌,表皮葡萄球菌和解糖链球菌)和链球菌属。 (无乳链球菌,中间链球菌,变形链球菌,血红球菌I和血红球菌II)。只有Peptostreptococcus anaerobius菌株在MNT肉汤培养物中产生己酸和戊酸。所有11个厌氧假单胞菌菌株在MNT肉汤中均产生戊酸,只有1个菌株未能在培养基中产生己酸。这项独特的功能有助于通过最少的测试快速,可靠地鉴定假单胞菌。

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