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首页> 外文期刊>Journal of Clinical Microbiology >Comparative chemotaxonomic studies of mycolic acid-free coryneform bacteria of human origin.
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Comparative chemotaxonomic studies of mycolic acid-free coryneform bacteria of human origin.

机译：人类起源的无霉菌酸的棒状细菌的比较化学分类学研究。

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Forty-two clinical isolates were classified as Corynebacterium minutissimum, Corynebacterium striatum, and Corynebacterium CDC group I by the API Coryne system. The chemotaxonomic characteristics of the isolates were determined by thin-layer chromatographic analysis. Twenty-six isolates were found to have a type IV cell wall (meso-di-aminopimelic acid arabinose, galactose) but did not contain mycolic acids. These 26 isolates shared chemotaxonomic characteristics with those of mycolic acid-free reference strains (including the Corynebacterium amycolatum NCFB 2768 type strain, "Corynebacterium asperum," and coryneform CDC groups I2 and F2). The total protein profiles of the isolates determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were similar to each other and to that of the C. amycolatum type strain. The profiles of the reference strains "Corynebacterium asperum" (CIP 100836, CIP 80.54, CIP 79.37, CIP 52.13), coryneform bacteria CDC groups I2 and F2 (CDC F5771, F5890, G723, G1970), and C. amycolatum were closely related. Thus, the mycolic acid-negative strains with a chemotype IV wall may belong to a single taxon. DNA hybridization studies could confirm this hypothesis. The present study shows the importance of chemotaxonomic analysis for verifying strain identifications and completing results from biochemical tests, particularly for coryneform bacteria.
机译：根据API Coryne系统，将42种临床分离株分为小棒杆菌，纹状棒杆菌和CDC第一类。分离物的化学分类学特征通过薄层色谱分析确定。发现有26种分离物具有IV型细胞壁（间-二氨基庚二酸阿拉伯糖，半乳糖），但不含霉菌酸。这26个分离株与无霉菌酸的参考菌株（包括棒状棒状杆菌NCFB 2768型菌株，“曲霉棒状杆菌”和棒状CDC I2和F2组）具有相同的化学分类学特征。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳确定的分离物的总蛋白谱彼此相似，与C.amycolatum型菌株相似。参考菌株“曲霉棒杆菌”（CIP 100836，CIP 80.54，CIP 79.37，CIP 52.13），棒状细菌CDC组I2和F2（CDC F5771，F5890，G723，G1970）和C.amycolatum的概况密切相关。因此，具有化学型IV壁的霉菌酸阴性菌株可以属于单一分类群。 DNA杂交研究可以证实这一假设。本研究表明化学分类学分析对于验证菌株鉴定和完成生化测试（尤其是棒状细菌）的结果的重要性。

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