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首页> 外文期刊>Journal of Clinical Microbiology >Quantitation of pneumococcal C polysaccharide in sputum samples from patients with presumptive pneumococcal pneumonia by enzyme immunoassay.
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Quantitation of pneumococcal C polysaccharide in sputum samples from patients with presumptive pneumococcal pneumonia by enzyme immunoassay.

机译：通过酶免疫法定量分析推定的肺炎球菌肺炎患者痰液中的肺炎球菌C多糖。

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Although the Gram stain and culture of expectorated sputum are considered standard methods for the diagnosis of presumptive pneumococcal pneumonia, these methods remain relatively insensitive and nonspecific. We developed an enzyme immunoassay (EIA) for the quantitation of pneumococcal C polysaccharide (PnC) in the sputum of patients with presumptive pneumococcal pneumonia. Of 34 patient sputum samples collected within 24 h of the first radiographic report of pneumonia, 12 grew Streptococcus pneumoniae on culture. By using a cutoff point of 0.5 micrograms of PnC per ml of sputum, all 12 specimens were positive (sensitivity, 100%) by EIA. PnC levels ranged from 1.43 to 57.53 micrograms/ml. Blood samples from 18 of the 34 patients were cultured. S. pneumoniae grew in the culture of a blood sample from one patient, whose sputum also had the highest PnC level. Of 22 sputum samples from patients with pneumonia that did not grow S. pneumonia, two were positive by EIA (specificity, 90.1%). Sputa from both patients had low levels of PnC (2.7 and 4.5 micrograms/ml), and both patients had received antibiotics before sputum collection. The positive predictive value of the quantitative EIA was 85.7%. Quantitation of PnC has the potential for improving the accuracy of sputum examination for S. pneumoniae, monitoring disease severity and the effectiveness of antibiotic therapy, and differentiating between those patients with invasive pneumococcal disease and those who are carriers of S. pneumoniae.
机译：尽管革兰氏染色和痰标本培养被认为是诊断推定性肺炎球菌性肺炎的标准方法，但这些方法仍然相对不敏感且没有特异性。我们开发了一种酶联免疫测定（EIA），用于定量推定肺炎球菌性肺炎患者痰液中的肺炎球菌C多糖（PnC）。在首次获得肺炎影像学报告后24小时内收集的34例患者痰标本中，有12例在培养中生长了肺炎链球菌。通过使用每毫升痰液0.5微克PnC的临界点，所有12个样本的EIA均为阳性（灵敏度为100％）。 PnC水平范围为1.43至57.53微克/毫升。培养了34名患者中18名的血样。一名患者的血液样本中培养了肺炎链球菌，其痰中PnC含量也最高。在22例未生长出肺炎链球菌的肺炎患者的痰标本中，有2例经EIA呈阳性（特异性为90.1％）。两名患者的痰液中PnC含量均较低（分别为2.7和4.5微克/毫升），并且两名患者在收集痰液之前均已接受了抗生素治疗。定量EIA的阳性预测值为85.7％。 PnC的定量分析具有提高肺炎链球菌痰检准确性，监测疾病严重程度和抗生素治疗效果以及区分浸润性肺炎球菌病患者和肺炎链球菌携带者的潜力。

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