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首页> 外文期刊>Journal of Clinical Microbiology >Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus.
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Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus.

机译：脉冲场凝胶电泳可替代金黄色葡萄球菌的噬菌体分型。

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Bacteriophage typing (BT) (World Health Organization method) has been used at the Centers for Disease Control and Prevention for over 30 years to type isolates of Staphylococcus aureus. Since studies have shown that BT patterns have poor reproducibility and because BT fails to type a high percentage (15 to 20%) of isolates, the Centers for Disease Control and Prevention has converted from using BT to using pulsed-field gel electrophoresis (PFGE) for strain typing S. aureus. We compared the results of BT with results of PFGE for typing 300 isolates of S. aureus, including strains from several well-characterized outbreaks. Ninety-six isolates were BT group I, 19 were group II, 82 were group III, 7 were group V, and 96 were nontypeable. PFGE identified subgroups within each phage group and thus was more discriminating than BT, which identified no subgroups. PFGE was able to type all isolates and distinguish related from unrelated strains of S. aureus. Our modified, standardized PFGE methodology should enable typing laboratories to obtain rapid, reliable results in 3 to 4 days when starting with an isolated colony on agar media.
机译：噬菌体分型（BT）（世界卫生组织方法）已在疾病控制和预防中心使用了30多年，用于分型金黄色葡萄球菌。由于研究表明BT模式的重现性较差，并且由于BT无法键入高比例（15％至20％）的分离株，因此疾病控制与预防中心已从使用BT转变为使用脉冲场凝胶电泳（PFGE）用于金黄色葡萄球菌的菌株分型。我们比较了BT和PFGE对300株金黄色葡萄球菌的分型结果，包括来自几个特征明确的暴发的菌株。 BT组I共有96个分离株，II组19个，III组82个，V组7个，不可分型96个。 PFGE鉴定了每个噬菌体组内的亚组，因此比BT（没有鉴定亚组）更具区分性。 PFGE能够对所有分离株进行分型，并区分出无关的金黄色葡萄球菌菌株。当从琼脂培养基上的分离菌落开始时，我们经过改进的标准化PFGE方法应可使打字实验室在3到4天内获得快速，可靠的结果。

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