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首页> 外文期刊>Journal of Clinical Microbiology >Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil.
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Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil.

机译：流行性多耐药金黄色葡萄球菌克隆在巴西的地理分布。

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Staphylococcus aureus isolates from five large teaching hospitals and one medium-size community hospital located in geographically distant parts of Brazil, in the south and southeast (Rio de Janeiro, Niteroi, Sao Paulo, Porto Alegre) and in the north (Manaus), were tested for their antibiotic resistance patterns and genetic backgrounds. Eighty-five of the 152 isolates were identified as methicillin-resistant S. aureus (MRSA) by using a combination of an agar dilution screen and a mecA gene-specific DNA probe. All MRSA isolates were resistant to penicillin, erythromycin, gentamicin, oxacillin, and cephalothin, and the majority of isolates (74%) were also resistant to chloramphenicol, sulfamethoxazole-trimethoprim, ciprofloxacin, and clindamycin as well and were susceptible only to vancomycin. Isolates obtained from hospitals in Sao Paulo, Rio de Janeiro, Niteroi, and Porto Alegre (1,600 km from one another) and Manaus (3,700 km from Rio de Janeiro) were examined by a variety of molecular fingerprinting techniques: the nature of the mecA polymorph and Tn554 attachment sites and restriction fragment length polymorphism of genomic DNAs after SmaI restriction and separation of the digested DNA by pulsed-field gel electrophoresis. The overwhelming majority of the isolates shared a common pulsed-field gel electrophoresis pattern and carried mecA polymorph III in combination with Tn554 pattern B, indicating the presence of a single, epidemic MRSA clone spread over large geographic distances of Brazil.
机译：金黄色葡萄球菌来自巴西的地理偏远地区，南部和东南部（里约热内卢，尼泰罗伊，圣保罗，阿雷格里港）和北部（马瑙斯）的五家大型教学医院和一家中型社区医院。测试其抗生素耐药性模式和遗传背景。通过使用琼脂稀释筛选和mecA基因特异性DNA探针的组合，在152个分离物中有85个被鉴定为耐甲氧西林金黄色葡萄球菌（MRSA）。所有MRSA分离株均对青霉素，红霉素，庆大霉素，奥沙西林和头孢菌素具有抗药性，大多数分离株（74％）对氯霉素，磺胺甲恶唑-甲氧苄啶，环丙沙星和克林霉素也有抗药性，仅对万古霉素敏感。通过多种分子指纹技术检查了从圣保罗，里约热内卢，尼泰罗伊和阿雷格里港（彼此相距1600公里）和马瑙斯（距里约热内卢3700公里）的医院获得的分离株：mecA多晶型物的性质SmaI限制和脉冲场凝胶电泳分离消化的DNA后，基因组DNA的Tn554和Tn554附着位点和限制片段长度多态性。绝大多数分离株具有共同的脉冲场凝胶电泳图谱，并带有mecA多晶型物III和Tn554图谱B，表明存在单个流行的MRSA克隆，分布在巴西很大的地理距离上。

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