Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6 isolates revealed by whole-cell repetitive element sequence-based PCR.

C L Poh; V Ramachandran; J W Tapsall

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Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6 isolates revealed by whole-cell repetitive element sequence-based PCR.

机译：基于全细胞重复元件序列的PCR揭示淋病奈瑟氏球菌IB-2和IB-6分离株的遗传多样性。

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Phenotypic characterization of 19 Neisseria gonorrhoeae serovar IB-2 and 8 serovar IB-6 isolates by the combined use of auxotypes, serological characterization, and penicillin susceptibility testing indicated intraserovar genetic diversity. In the present study, we applied whole-cell repetitive element sequence-based PCR (rep-PCR) analysis which allows a rapid assessment of the clonal relationships of IB-2 and IB-6 isolates. DNA templates were prepared by boiling cells harvested directly from plate cultures, eliminating the need for time-consuming phenol extraction. Six different rep-PCR profiles were established among the 19 IB-2 isolates. Rep-PCR typing results had a good correlation with pulsed-field gel electrophoresis patterns. It is slightly less discriminatory than BglII-generated macrorestriction pattern analysis by pulsed-field gel electrophoresis. It is capable of discriminating epidemiologically related from epidemiologically unrelated IB-2 isolates. It should serve as a rapid and useful subtyping tool for epidemiologic investigations in which there is a predominance of major serovar groups.

机译：19型淋病奈瑟氏球菌IB-2和8血清型IB-6分离株的表型鉴定通过结合使用拟型，血清学鉴定和青霉素敏感性测试表明了血清内遗传多样性。在本研究中，我们应用了基于全细胞重复元件序列的PCR（rep-PCR）分析，该分析可以快速评估IB-2和IB-6分离株的克隆关系。通过煮沸直接从平板培养物中收获的细胞来制备DNA模板，从而无需费时的苯酚提取。在19个IB-2分离株中建立了六个不同的rep-PCR图谱。 Rep-PCR分型结果与脉冲场凝胶电泳图谱有很好的相关性。它与通过脉冲场凝胶电泳进行的BglII生成的宏观限制性酶切图谱分析相比，具有较低的歧视性。它能够区分与流行病学无关的IB-2分离株。它应该作为流行病学调查的快速和有用的亚型工具，在这些流行病学调查中，主要的血清型群占多数。

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《Journal of Clinical Microbiology》 |1996年第2期|共4页
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C L Poh; V Ramachandran; J W Tapsall;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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机译：基于全细胞重复元件序列的聚合酶链反应检测到的霍乱弧菌O1和非O1分离株DNA序列的多样性
2. Multilocus sequence typing and repetitive element-based polymerase chain reaction analysis of Neisseria meningitidis isolates in Brazil reveal the emergence of 11 new sequence types genetically related to the ST-32 and ST-41/44 complexes and high pre [J] . de Filippis I, Vicente AC Diagnostic microbiology and infectious disease . 2005,第3期

机译：巴西脑膜炎奈瑟氏球菌分离株的多基因座序列分型和基于重复元素的聚合酶链反应分析显示，出现了11种与ST-32和ST-41 / 44复合物及高抗
3. Whole-cell repetitive element sequence-based polymerase chain reaction allows rapid assessment of clonal relationships of bacterial isolates. [J] . C R Woods, J Versalovic, T Koeuth, Journal of Clinical Microbiology . 1993,第7期

机译：基于全细胞重复元件序列的聚合酶链反应可快速评估细菌分离株的克隆关系。
4. Sequence-based Deep Learning Reveals the Bacterial Community Diversity and Horizontal Gene Transfer [C] . Hao Zheng, Jie Yin, Jieruo Gu, International Conference on Bioinformatics and Computational Biology . 2017

机译：基于序列的深度学习揭示了细菌群落多样性和水平基因转移
5. Genetic and biochemical analysis of NsrR, a nitric oxide-responsive repressor in Neisseria gonorrhoeae. [D] . Isabella, Vincent Michael. 2010

机译：淋病奈瑟氏球菌一氧化氮反应阻遏物NsrR的遗传和生化分析。
6. Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6 isolates revealed by whole-cell repetitive element sequence-based PCR. [O] . C L Poh, V Ramachandran, J W Tapsall 1996

机译：基于全细胞重复元件序列的PCR揭示淋病奈瑟氏球菌IB-2和IB-6分离株的遗传多样性。
7. Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6 isolates revealed by whole-cell repetitive element sequence-based PCR [O] . C L Poh, V Ramachandran, J W Tapsall 1996

机译：Neisseria淋病IB-2和IB-6分离物的遗传多样性，由全细胞重复元素序列的PCR透露
8. Susceptibility of Neisseria gonorrhoeae Isolates from Jakarta, Indonesia, to Antibiotics. [R] . Rockhill, R. C., Leksana, B., Wijaya, U., 1982

机译：从印度尼西亚雅加达到抗生素的淋病奈瑟氏球菌的易感性。

Genetic diversity of Neisseria gonorrhoeae IB-2 and IB-6 isolates revealed by whole-cell repetitive element sequence-based PCR.

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