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首页> 外文期刊>Journal of Clinical Microbiology >Rapid Immunoassay for detection of Escherichia coli O157 directly from stool specimens.
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Rapid Immunoassay for detection of Escherichia coli O157 directly from stool specimens.

机译：直接从粪便标本中快速检测大肠杆菌O157的快速免疫分析。

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A new and rapid ( < 1 h) enzyme-linked immunosorbent assay (ELISA) was compared with conventional sorbitol-MacConkey agar (SMAC) culture for the detection of Escherichia coli O157 from stool specimens. Among 34 positive specimens, confirmed by colony-sweeping and immunofluorescence stain methods, 6 did not exhibit visible sorbitol-negative colonies on SMAC. These six specimens would have been considered to be negative if SMAC alone had been used. The ELISA detected 31 of the 34 positive samples, including 5 of the above-mentioned 6 false-negative samples, resulting in a sensitivity and specificity of 91.2 and 99.5%, respectively. Cross-reactivity with other enteric pathogens was not noted by ELISA. The SMAC method had a sensitivity and specificity of 82.4 and 100%, respectively. The ELISA-negative specimens do not require culture confirmation, whereas positive results must be considered to be presumptive until confirmed by culture. The test is accurate and is easy to perform, making it a very efficient method for screening stool specimens for E. coli O157.
机译：一种新的和快速（<1小时）酶联免疫吸附测定（ELISA）与常规山梨糖醇-MacConkey琼脂（SMAC）培养进行了比较，用于从粪便标本中检测大肠杆菌O157。在34个阳性标本中，通过集落扫描和免疫荧光染色法确认，其中6个在SMAC上未显示可见的山梨醇阴性菌落。如果仅使用SMAC，则这六个样本将被视为阴性。 ELISA检测到34个阳性样品中的31个，包括上述6个假阴性样品中的5个，敏感性和特异性分别为91.2和99.5％。 ELISA未发现与其他肠道病原体的交叉反应。 SMAC方法的灵敏度和特异性分别为82.4和100％。 ELISA阴性标本不需要培养确认，而阳性结果在被培养确认之前必须被认为是推测性的。该测试准确且易于执行，使其成为筛查粪便标本中大肠杆菌O157的非常有效的方法。

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