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首页> 外文期刊>Journal of Clinical Microbiology >Study of the Relatedness of Isolates ofShigella flexneri and Shigella sonnei Obtained in 1986 and 1987 and in 1994 and 1995 from Hong Kong
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Study of the Relatedness of Isolates ofShigella flexneri and Shigella sonnei Obtained in 1986 and 1987 and in 1994 and 1995 from Hong Kong

机译：1986年和1987年以及1994年和1995年从香港获得的弗氏志贺氏菌和索氏志贺氏菌分离株的相关性研究

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We used pulsed-field gel electrophoresis (PFGE) to study the genetic relatedness of 235 isolates of Shigella flexneriand Shigella sonnei collected in Hong Kong (97 isolates from 1986 and 1987 and 138 isolates from 1994 and 1995). Altogether, 13 gels were run with bacteriophage lambda ladder DNA (Pharmacia) as an external reference in every sixth lane, standardized reagents and methods, and isolates randomized for species and years. For quantitative illustration of the relationships within a large body of isolates, computer-generated dendrograms were used to determine the number of isolates in pulsotypes at Dice coefficients of similarity of 75% (PT75) and 50% (PT50). For S. flexneri, there was a significant difference in the distribution of isolates collected during the two periods in both PT75and PT50, with 68% of isolates collected in 1994 and 1995 sharing a coefficient of similarity of ≥68%. For S. sonnei, a significant difference was observed in PT50only. We also used Upholt’s formula for an approximation of the fraction of nucleotide difference between isolates and Molecular Evolutionary Genetics Analysis to determine relative genetic distances. For both species, the relative genetic distances between isolates of the earlier collection period were significantly greater (P < 0.0001), i.e., they were further apart and therefore more diverse than those of the later period. We conclude that it is possible for a typical clinical laboratory to analyze a large amount of PFGE information on Shigella isolates obtained under controlled conditions. Such data analysis should enhance surveillance capabilities and give indications of further work to be done on various aspects of bacterial pathogenicity of the species.
机译：我们使用脉冲场凝胶电泳（PFGE）研究了在香港收集的235株志贺氏菌和志贺氏菌的遗传相关性（1986和1987年有97株（1994和1995年的138株）。在第六个泳道中，共使用13个凝胶以噬菌体λ梯形DNA（Pharmacia）作为外部参考，使用标准化的试剂和方法，并按物种和年份随机分配了分离物。为了定量说明大量分离株之间的关系，计算机生成的树状图用于确定Dice相似系数分别为75％（PT 75 ）和50％（Dice）的脉冲型分离株的数量。 PT 50 ）。对于 S。 flexneri ，在两个时期内，PT 75 和PT 50 中分离株的分布存在显着差异，其中68％分离株在1994年和1995年的相似系数≥68％。对于 S。 sonnei ，仅PT 50 观察到显着差异。我们还使用Upholt公式对分离株和分子进化遗传学分析之间的核苷酸差异分数进行了近似计算，以确定相对遗传距离。对于这两个物种，早期采集菌株之间的相对遗传距离都明显更大（ P <0.0001），也就是说，它们之间的距离较远，因此比后期采集的物种更多样化。我们得出的结论是，对于典型的临床实验室来说，有可能分析在受控条件下获得的志贺氏菌分离株的大量PFGE信息。此类数据分析应增强监视能力，并为进一步研究该物种细菌致病性的各个方面提供指示。

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