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首页> 外文期刊>Journal of Clinical Microbiology >Performance of Transcription-Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods
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Performance of Transcription-Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods

机译：转录介导的扩增和连接酶链反应检测在有创和无创方法获得的泌尿生殖道标本中衣原体感染检测中的性能

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Based on the amplification of chlamydia-specific rRNA sequences and the ligase chain reaction (LCR), the performance characteristics of the Gen-Probe Chlamydia trachomatistranscription-mediated amplification (TMA) assay were evaluated with endocervical, urine, and vulval specimens from women and urethral and urine specimens from men and were compared with those for cultures on endocervical, vulval, and urethral swabs. Of the 308 women and 240 men tested, 25 (8.1%) and 44 (18.3%), respectively, were shown to be infected. By using the infected individual as the expanded “gold standard” for calculations, the TMA assay and LCR gave similar performances for the sensitivity of male urethral (93.2%) and urine (88.6 and 86.4%) samples, while culture detected only half of the 44 infected men. In women, the sensitivities of the TMA assay for endocervical and vulval samples were 88 and 92%, respectively, compared to values of 92% for the LCR on both sample types and of 52 and 8%, respectively, for culture. By using first-void urine for chlamydial diagnosis in women, LCR detected 24 (96%) and TMA assay detected 19 (76%) infected individuals, showing a significantly lower sensitivity for urine in women (P = 0.0253). The results indicate a high overall agreement for both amplifying techniques for all examined specimen types, except for female urine. Furthermore, they confirm the previous observation that vulval swabs are an effective alternative noninvasive sample type for the detection of C. trachomatis infection in women by nucleic acid-based amplification technologies.
机译：基于衣原体特异性rRNA序列的扩增和连接酶链反应（LCR），用宫颈内膜，尿液评估了Gen-Probe沙眼衣原体转录介导扩增（TMA）检测的性能特点，以及女性的外阴标本，男性的尿道和尿液标本，以及与宫颈，外阴和尿道拭子上培养物的标本进行了比较。在测试的308名女性和240名男性中，分别有25名（8.1％）和44名（18.3％）被感染。通过使用感染的个体作为扩展的“金标准”进行计算，TMA测定法和LCR对男性尿道样本（93.2％）和尿液（88.6％和86.4％）的敏感性表现出相似的表现，而培养液仅检测到一半。 44名感染者。在女性中，TMA测定法对宫颈和外阴样本的敏感性分别为88％和92％，而两种类型的LCR的敏感度分别为92％和0.5％。通过使用初次排尿进行女性衣原体诊断，LCR检测出24（96％），TMA分析检测出19（76％）感染个体，表明女性对尿液的敏感性明显降低（ P = 0.0253）。结果表明，除了女性尿液外，所有扩增样本都适用于两种扩增技术。此外，他们证实了先前的观察，即外阴拭子是检测 C的有效替代性非侵入性样本类型。核酸扩增技术在女性沙眼感染中的应用

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