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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Neutralizing Antibodies against Human Papillomaviruses (HPV) by Inhibition of Gene Transfer Mediated by HPV Pseudovirions
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Detection of Neutralizing Antibodies against Human Papillomaviruses (HPV) by Inhibition of Gene Transfer Mediated by HPV Pseudovirions

机译：通过抑制HPV伪病毒介导的基因转移来检测抗人乳头瘤病毒（HPV）的中和抗体

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The goal of this study was to develop a human papillomavirus (HPV) neutralization assay using HPV pseudovirions generated in vitro. For this purpose, gene transfer efficiency of HPV virus-like particles (VLPs) was improved by using direct interaction between a reporter plasmid and the VLPs. Electron microscopic observation of the interaction between DNA molecules and VLPs revealed that VLPs always interact with a single DNA molecule and that VLPs bind to the end of linearized DNA molecules. An 100-fold improvement in the gene transfer was obtained by simple interaction between a linearized DNA molecule and VLPs. Moreover, direct interaction methods offer the possibility of transferring plasmids a size higher than that of the papillomavirus genome. The approach that we developed to generate HPV-16 and HPV-31 pseudovirions proved to be suitable for testing neutralizing antibodies in human sera both after immunization and after natural infection.
机译：这项研究的目的是使用体外产生的HPV假病毒粒子开发人乳头瘤病毒（HPV）中和试验。为此，通过利用报告质粒和VLP之间的直接相互作用来提高HPV病毒样颗粒（VLP）的基因转移效率。用电子显微镜观察DNA分子和VLP之间的相互作用，发现VLP始终与单个DNA分子相互作用，并且VLP结合到线性化DNA分子的末端。通过线性化的DNA分子和VLP之间的简单相互作用，基因转移提高了100倍。此外，直接相互作用方法提供了转移质粒的可能性，该质粒的大小大于乳头瘤病毒基因组的大小。我们开发的产生HPV-16和HPV-31假病毒粒子的方法被证明适用于在免疫后和自然感染后测试人血清中的中和抗体。

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