Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates

N. Galí; J. Domínguez; S. Blanco; C. Prat; M. D. Quesada; L. Matas

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Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates

机译：基于分枝杆菌噬菌体的室内检测法在结核分枝杆菌临床分离株中快速检测利福平耐药性的实用性

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摘要

A rapid in-house mycobacteriophage-based assay to identify multidrug resistance by detecting the rifampin susceptibility of Mycobacterium tuberculosis in a microtiter plate format was evaluated. The sensitivity, specificity, and overall accuracy of the assay were 100%. This test is rapid to perform and suitable for widespread implementation.

机译：评估了一种快速的内部分枝杆菌噬菌体检测方法，通过检测微量滴定板形式的结核分枝杆菌的利福平敏感性来鉴定多药耐药性。测定的灵敏度，特异性和整体准确性为100％。该测试执行迅速且适合广泛实施。 展开▼

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《Journal of Clinical Microbiology》 |2003年第6期|共3页

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N. Galí; J. Domínguez; S. Blanco; C. Prat; M. D. Quesada; L. Matas;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Evaluation of Reverse Transcription-PCR and a Bacteriophage-Based Assay for Rapid Phenotypic Detection of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosis [J] . I. J. Eltringham, F. A. Drobniewski, J. A. Mangan, Journal of Clinical Microbiology . 1999,第11期

机译：逆转录PCR和基于噬菌体的快速评估表型结核分枝杆菌临床分离株对利福平耐药性的评估。

2. Use of a Mycobacteriophage-Based Assay for Rapid Assessment of Susceptibilities of Mycobacterium tuberculosis Isolates to Isoniazid and Influence of Resistance Level on Assay Performance [J] . N. Galí, J. Domínguez, S. Blanco, Journal of Clinical Microbiology . 2006,第1期

机译：基于分枝杆菌噬菌体的测定法快速评估结核分枝杆菌分离物对异烟肼的敏感性以及耐药水平对测定性能的影响

3. Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Isolates by Heteroduplex Analysis and Determination of Rifamycin Cross-Resistance in Rifampin-Resistant Isolates [J] . Zeynep Sar?ba?, Tan?l Kocag?z, Alpaslan Alp, Journal of Clinical Microbiology . 2003,第2期

机译：异源双链分析法快速检测结核分枝杆菌分离株对利福平的耐药性及耐利福平分离株中利福霉素交叉耐药性的测定

4. Molecular Genetic Analysis of Nucleotide Polymorphisms Associated with Rifampin and Isoniazid Resistance in Human Isolates of Mycobacterium tuberculosis [C] . Dorina Banica, Emilia Cazacu, Dan Otelea, International Symposium on Communications, Control and Signal Processing . 2008

机译：分枝杆菌结核病人分离株核苷酸多态性核苷酸多态性的分子遗传分析

5. Characterization of Fluoroquinolone Resistance in Mycobacterium tuberculosis Clinical Isolates Using Molecular Dynamics and Targeted Genome Wide Variant Analysis [D] . Amallraja, Anu. 2018

机译：结核分枝杆菌临床分离株中氟喹诺酮耐药性的分子动力学和靶向基因组广泛变异分析

6. Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates [O] . N. Galí, J. Domínguez, S. Blanco, 2003

机译：基于分枝杆菌噬菌体的室内检测方法在结核分枝杆菌临床分离株中快速检测利福平耐药性的实用性

7. Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates [O] . Galí, N., Domínguez, J., Blanco, S., 2003

机译：基于分枝杆菌噬菌体的室内检测方法在结核分枝杆菌临床分离株中快速检测利福平耐药性的实用性

1. MTBDR plus方法快速检测北京地区临床结核分枝杆菌分离株利福平和异烟肼耐药性效果评价 [J] . 菅记涌 ,王嫩寒 ,易俊莉 . 中国防痨杂志 . 2010,第010期

2. 结核分枝杆菌临床分离株利福平和异烟肼耐药性检测方法比较 [J] . 李同霞 ,江国峰 ,郝文嘉 . 中国现代医学杂志 . 2016,第024期

3. 微孔板Alamar Blue显色法检测结核分枝杆菌临床分离株利福平和异烟肼耐药性研究 [J] . 李桂莲 ,张敬蕊 ,赵秀芹 . 中国人兽共患病学报 . 2012,第004期

4. 高分辨率熔解曲线技术用于结核分枝杆菌临床分离株异烟肼耐药性的快速检测 [J] . 杨彩虹 ,杨敏 ,于璐 . 中国人兽共患病学报 . 2017,第005期

5. 多中心评价逆向系统印迹分析在临床结核分枝杆菌分离株耐药性检测中的应用 [J] . 张健源 . 结核病与胸部肿瘤 . 2004,第003期

6. MTBDR plus方法快速检测北京地区临床结核分枝杆菌分离株利福平和异烟肼耐药性效果评价 [C] . 菅记涌 ,王嫩寒 ,易俊莉 . 2010年中国防痨协会临床/基础专业学术大会 . 2010

7. 石家庄地区结核分枝杆菌临床分离株基因分型及耐药性分析 [A] . 张志 . 2015

1. 基于CRISPR-Cas技术检测结核分枝杆菌利福平耐药性点突变的crRNA [P] . 中国专利： CN114107300A . 2022-03-01

2. 新疆地区的结核分枝杆菌临床分离株中存在PhoP基因的天然缺失突变菌株 [P] . 中国专利： CN109810932A . 2019-05-28

3. Detection method and kit for simultaneously detecting the detection and identification of tuberculosis and non-tuberculous mycobacteria based on the Quanta Matrix Assay platform and detection of quiescent tuberculosis antibiotics and quanta matrix assay platforms and identification of rifampin resistance of Mycobacterium tuberculosis [P] . 外国专利： KR20190022871A . 2019-03-06

机译：基于Quanta Matrix Assay平台同时检测结核和非结核分枝杆菌的检测与鉴定，静态结核菌抗生素和Quanta矩阵检测平台的检测以及结核分枝杆菌对利福平抗性的鉴定的检测方法和试剂盒

4. DIAGNOSTIC METHOD CAPABLE OF SIMULTANEOUSLY CONFIRMING DETECTION OF NONTUBERCULOUS MYCOBACTERIA AND MYCOBACTERIUM TUBERCULOSIS AND RESISTANCE TO RIFAMPIN, ISONIAZID, FLUOROQUINOLONES, AND AMINOGLYCOSIDE ANTIBIOTICS OF MYCOBACTERIUM TUBERCULOSIS, AND A KIT THEREOF, BASED ON QUANTA MATRIX ASSAY PLATFORM [P] . 外国专利： KR101962831B1 . 2019-03-27

机译：能够同时确认非结核性分枝杆菌和结核分枝杆菌的诊断方法以及对利福平，异烟酰胺，氟喹诺酮和氨糖苷类抗生素，霉菌毒素和抗生素的耐药性

5. IMPROVED QUANTAMATRIX ASSAY PLATFORM-BASED, DIAGNOSTIC METHOD CAPABLE OF SIMULTANEOUSLY IDENTIFYING DETECTION OF MYCOBACTERIUM TUBERCULOSIS AND RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS TO RIFAMPIN, ISONIAZID, ETHAMBUTOL, QUINOLONE, SECOND LINE INJECTABLE DRUGS SUCH AS AMIKACIN, KANAMYCIN, AND CAPEOMYCIN, AND STREPTOMYCIN DRUGS, AND KIT THEREFOR [P] . 外国专利： WO2019225959A1 . 2019-11-28

机译：基于改进的量子矩阵分析平台的诊断方法，能够同时鉴定结核分枝杆菌的结核分枝杆菌，以及对结核分枝杆菌对利福平，异烟肼，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇，肌醇和肌醇那个

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Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates

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