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首页> 外文期刊>Journal of Clinical Microbiology >Direct Isolation of Candida spp. from Blood Cultures on the Chromogenic Medium CHROMagar Candida
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Direct Isolation of Candida spp. from Blood Cultures on the Chromogenic Medium CHROMagar Candida

机译：直接分离假丝酵母。色培养基CHROMagar Candida上的血液培养物分离

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CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast directly from clinical specimens, including stool, urine, respiratory, vaginal, oropharyngeal, and esophageal sources. Direct isolation of yeast on CHROMagar Candida from blood cultures has not been evaluated. We evaluated whether the color and colony characteristics produced by Candida spp. on CHROMagar Candida were altered when yeasts were isolated directly from blood cultures. Fifty clinical isolates of Candida were inoculated into aerobic and anaerobic blood culture bottles and incubated at 35°C in an automated blood culture system. When growth was detected, an aliquot was removed and plated onto CHROMagar Candida. As a control, CHROMagar Candida plates were inoculated with the same isolate of yeast grown on Sabouraud dextrose agar simultaneously. No significant difference was detected in color or colony morphology between the blood and control isolates in any of the tested organisms. All C. albicans (n = 12), C. tropicalis (n = 12), C. glabrata (n = 9), and C. krusei (n = 5) isolates exhibited the expected species-specific colony characteristics and color, whether isolated directly from blood or from control cultures. CHROMagar Candida can be reliably used for direct isolation of yeast from blood cultures. Direct isolation could allow mycology laboratories to more rapidly identify Candida spp., enable clinicians to more quickly make antifungal agent selections, and potentially decrease patient morbidity and mortality.
机译：CHROMagar Candida是一种选择性的差异显色培养基，已证明可用于鉴定白色念珠菌，克鲁斯念珠菌，热带念珠菌和也许是 gladata 。当CHROMagar念珠菌已用于从临床标本中直接分离酵母时，菌落的形态和颜色已得到很好的定义，临床标本包括粪便，尿液，呼吸道，阴道，口咽和食道来源。尚未评估从血液培养物中直接分离CHROMagar念珠菌上的酵母。我们评估了 Candida spp产生的颜色和菌落特性。直接从血液培养物中分离酵母时，CHROMagar念珠菌的抗性改变。将五十株 Candida 临床分离株接种到需氧和厌氧的血培养瓶中，并在自动血培养系统中于35°C孵育。当检测到生长时，取出等分试样并铺在CHROMagar假丝酵母上。作为对照，用在Sabouraud葡萄糖琼脂上同时生长的酵母的相同分离株接种CHROMagar假丝酵母平板。在任何测试的生物体中，血液和对照分离株之间在颜色或菌落形态上均未检测到显着差异。所有 C。白色（（ n = 12）， C。 Tropicalis （ n = 12）， C。 glabrata （ n = 9）和 C。无论是直接从血液还是从对照培养物中分离得到的krusei （ n = 5）分离株均具有预期的物种特异性菌落特征和颜色。 CHROMagar念珠菌可以可靠地用于从血液培养物中直接分离酵母。直接隔离可以使真菌学实验室更快地鉴定出 Candida 菌种，使临床医生可以更快地选择抗真菌药，并有可能降低患者的发病率和死亡率。

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