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首页> 外文期刊>Journal of Clinical Microbiology >PCR Assay To Detect Bacillus anthracis Spores in Heat-Treated Specimens
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PCR Assay To Detect Bacillus anthracis Spores in Heat-Treated Specimens

机译:PCR方法检测热处理样品中的炭疽芽孢杆菌孢子

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Recent interest in anthrax is due to its potential use in bioterrorism and as a biowarfare agent against civilian populations. The development of rapid and sensitive techniques to detect anthrax spores in suspicious specimens is the most important aim for public health. With a view to preventing exposure of laboratory workers to viable Bacillus anthracis spores, this study evaluated the suitability of PCR assays for detecting anthrax spores previously inactivated at 121°C for 45 min. The results indicate that heat treatment ensures the complete inactivation of B. anthracis spores without significantly affecting the efficiency of PCR assays.
机译:最近对炭疽热的兴趣是由于其可用于生物恐怖主义和作为针对平民的生物战剂。快速,灵敏的技术来检测可疑标本中的炭疽芽孢是公共卫生的最重要目标。为了防止实验室工作人员接触活的炭疽芽孢杆菌孢子,本研究评估了PCR检测法检测先前在121°C灭活45分钟的炭疽芽孢的适用性。结果表明,热处理确保了 B的完全失活。炭疽菌孢子,而不会显着影响PCR分析的效率。

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