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首页> 外文期刊>Journal of Clinical Microbiology >Strain variation among and antifungal susceptibilities of isolates of Candida krusei.
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Strain variation among and antifungal susceptibilities of isolates of Candida krusei.

机译：克鲁斯假丝酵母菌株之间的菌株变异和抗真菌药敏性。

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Candida krusei is an emerging pathogen that is well known for its propensity to develop resistance to fluconazole and other azoles. Despite the potential clinical significance of C. krusei, little is known of its epidemiology and genetic diversity as defined by the newer DNA-based typing methods. We investigated the genotypic diversity and antifungal susceptibility of 67 clinical isolates from 44 patients and 5 health care workers from six different medical centers. Strain delineation was performed by restriction endonuclease analysis of genomic DNA (REAG) with the restriction enzyme HinfI followed by conventional electrophoresis. The susceptibility of the isolates to the antifungal agents amphotericin B, flucytosine, fluconazole, and itraconazole was determined by methods recommended by the National Committee for Clinical Laboratory Standards. The MICs at which 90% of the isolates were inhibited ranged from 1.0 microgram/ml for itraconazole to 64 micrograms/ml for fluconazole. In general, isolates from a given patient, or epidemiologically related isolates from a single institution, were identical by molecular typing methods. Epidemiologically unrelated isolates were distinctly different by the REAG typing method employed. These data document the genetic diversity and antifungal susceptibility of clinical isolates of C. krusei.
机译：克鲁斯假丝酵母是一种新兴病原体，以其对氟康唑和其他唑类产生耐药性而闻名。尽管克鲁氏梭菌具有潜在的临床意义，但对其流行病学和遗传多样性的了解却鲜为人知。我们调查了来自六个不同医学中心的44名患者和5名医护人员的67株临床分离株的基因型多样性和抗真菌药敏性。通过用限制酶HinfI对基因组DNA（REAG）进行限制性核酸内切酶分析，然后进行常规电泳来进行菌株鉴定。分离株对抗真菌药两性霉素B，氟胞嘧啶，氟康唑和伊曲康唑的敏感性通过国家临床实验室标准委员会推荐的方法确定。抑制90％分离物的MIC范围从伊曲康唑的1.0微克/毫升到氟康唑的64微克/毫升。通常，通过分子分型方法，来自给定患者的分离株或来自单个机构的流行病学相关分离株是相同的。流行病学无关的分离株通过采用的REAG分型方法明显不同。这些数据证明了克鲁氏梭菌临床分离株的遗传多样性和抗真菌药性。

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