Improvement of growth of Chlamydia pneumoniae on HEp-2 cells by pretreatment with polyethylene glycol in combination with additional centrifugation and extension of culture time.

J H Tjhie; R Roosendaal; D M MacLaren; C M Vandenbroucke-Grauls

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Improvement of growth of Chlamydia pneumoniae on HEp-2 cells by pretreatment with polyethylene glycol in combination with additional centrifugation and extension of culture time.

机译：通过用聚乙二醇预处理结合额外的离心作用和延长培养时间，可以改善肺炎衣原体在HEp-2细胞上的生长。

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The following adaptations led to improved growth of Chlamydia pneumoniae on HEp-2 cells compared to that by the standard method: monolayer preincubation with 7% polyethylene glycol (PEG), extension of incubation time to 7 days, and extension of incubation to 7 days in combination with centrifugation on days 3, 4, and 5. These adaptations resulted in approximate increases in numbers of inclusion-forming units (IFU) of 2-, 5-, and 69-fold, respectively. A combination of preincubation with PEG, prolonged incubation, and centrifugation on days 3, 4, and 5 increased the numbers of IFU >300-fold. This is therefore recommended as the optimal method for culturing C. pneumoniae.

机译：与标准方法相比，以下适应措施导致肺炎衣原体在HEp-2细胞上的生长得到改善：与7％聚乙二醇（PEG）进行单层预孵育，将孵育时间延长至7天，并在7d内将孵育延长至7天。在第3、4和5天进行离心分离。这些适应作用分别导致包裹形成单位（IFU）的数量分别增加了2倍，5倍和69倍。与PEG一起预孵育，延长孵育时间以及在第3、4和5天离心的组合增加了IFU的数量> 300倍。因此，建议将其作为培养肺炎衣原体的最佳方法。

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《Journal of Clinical Microbiology》 |1997年第7期|共2页
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J H Tjhie; R Roosendaal; D M MacLaren; C M Vandenbroucke-Grauls;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Involvement of Nicotinic Acetylcholine Receptors in Controlling Chlamydia pneumoniae Growth in Epithelial HEp-2 Cells [J] . Hiroyuki Yamaguchi, Herman Friedman, Yoshimasa Yamamoto Infection and immunity . 2003,第6期

机译：烟碱乙酰胆碱受体参与控制上皮HEp-2细胞中肺炎衣原体的生长。
2. Involvement of Nicotinic Acetylcholine Receptors in Controlling Chlamydia pneumoniae Growth in Epithelial HEp-2 Cells [J] . Hiroyuki Yamaguchi, Herman Friedman, Yoshimasa Yamamoto Infection and immunity . 2003,第6期

机译：烟碱乙酰胆碱受体参与控制上皮HEp-2细胞中肺炎衣原体的生长。
3. Lymphotoxin Inhibits Chlamydia pneumoniae Growth in HEp-2 Cells [J] . Hiroshi Matsushima, Mutsunori Shirai, Kazunobu Ouchi, Infection and immunity . 1999,第6期

机译：淋巴毒素抑制HEp-2细胞中的肺炎衣原体生长
4. Maximizing human induced pluripotent stem cell derived neural stem cell survival, axon extension and gene expression of neural differentiation markers using polyethylene glycol hydrogels containing a continuous concentration gradient in n-cadherin derived peptide His-Ala-Val-Asp-Lle [C] . Hyun Ju Lim, Zara Khan, T. Hiran Perera, Society for Biomaterials annual meeting and exposition . 2017

机译：使用在n-钙粘蛋白衍生肽His-Ala-Val-Asp-Lle中具有连续浓度梯度的聚乙二醇水凝胶，最大限度地提高人类诱导的多能干细胞衍生的神经干细胞的存活率，轴突延伸和神经分化标记物的基因表达
5. Improvement of growth of Chlamydia pneumoniae on HEp-2 cells by pretreatment with polyethylene glycol in combination with additional centrifugation and extension of culture time. [O] . J H Tjhie, R Roosendaal, D M MacLaren, 1997

机译：通过用聚乙二醇预处理结合额外的离心作用和延长培养时间可以改善肺炎衣原体在HEp-2细胞上的生长。
6. Involvement of Nicotinic Acetylcholine Receptors in Controlling Chlamydia pneumoniae Growth in Epithelial HEp-2 Cells [O] . Yamaguchi, Hiroyuki, Friedman, Herman, Yamamoto, Yoshimasa 2003

机译：烟碱型乙酰胆碱受体参与控制上皮HEp-2细胞中肺炎衣原体的生长。

Improvement of growth of Chlamydia pneumoniae on HEp-2 cells by pretreatment with polyethylene glycol in combination with additional centrifugation and extension of culture time.

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