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首页> 外文期刊>Journal of Clinical Microbiology >Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates.
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Relationship between adhesion to intestinal Caco-2 cells and multidrug resistance in Klebsiella pneumoniae clinical isolates.

机译：肺炎克雷伯菌临床分离株对肠道Caco-2细胞的粘附与多药耐药性之间的关系。

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Klebsiella pneumoniae is an opportunistic gram-negative pathogen involved in outbreaks of nosocomial infections in intensive care units. Strains are resistant to multiple antibiotics, and 15 to 30% of them are also resistant to the broad-spectrum cephalosporins by the production of R plasmid-encoded extended-spectrum beta-lactamases. Because the gastrointestinal tracts of patients have been shown to be the reservoir for nosocomial strains of K. pneumoniae, we looked for a correlation between antibiotic resistance and adhesion of K. pneumoniae strains to intestinal cells. We investigated adhesion to the human intestinal epithelial Caco-2 cell line of 61 clinical K. pneumoniae strains isolated in hospitals in Clermont-Ferrand, France. None of the strains tested expressed the previously described adhesive factors CF29K and KPF-28. Adhesive properties were found for 42.6% of the strains tested (26 strains). Just 7.7% (2 strains) of the 26 strains producing only the chromosomally encoded SHV-1 beta-lactamase adhered to the Caco-2 cell line, whereas 68.5% (24 strains) of the 35 strains producing a plasmid-encoded beta-lactamase were adherent. All the adherent strains, and even the two strains producing only the SHV-1 enzyme, harbored at least one self-transmissible R plasmid. At variance for CAZ-1/TEM-5 or CAZ-5/SHV-4 beta-lactamase-producing K. pneumoniae strains, curing and mating experiments demonstrated that the self-transmissible R plasmids encoding the TEM-1, CTX-1/TEM-3, CAZ-2/TEM-8, CAZ-6/TEM-24, or CAZ-7/TEM-16 beta-lactamase were not involved in the adhesion of K. pneumoniae strains to intestinal epithelial cells. Nevertheless, there was an association of multiple antibiotic resistance, including resistance to extended-spectrum cephalosporins, and adhesive properties in K. pneumoniae clinical isolates.
机译：肺炎克雷伯菌是一种机会性革兰氏阴性病原体，与重症监护病房的医院感染爆发有关。菌株对多种抗生素具有抗性，并且它们中的15％至30％通过产生R质粒编码的广谱β-内酰胺酶也对广谱头孢菌素具有抗性。由于已显示患者的胃肠道是肺炎克雷伯菌医院菌株的储藏库，因此我们寻找抗生素耐药性和肺炎克雷伯菌菌株对肠道细胞的粘附之间的相关性。我们调查了在法国克莱蒙费朗的医院中分离出的61株临床肺炎克雷伯菌菌株对人肠上皮Caco-2细胞系的粘附力。测试的菌株均未表达先前描述的粘附因子CF29K和KPF-28。对于测试的菌株（26个菌株）发现了42.6％的粘合性能。仅产生染色体编码的SHV-1β-内酰胺酶的26株菌株中只有7.7％（2株）粘附于Caco-2细胞系，而35株中68.5％（24株）产生质粒编码的β-内酰胺酶坚持。所有的粘附菌株，甚至是仅产生SHV-1酶的两种菌株，都具有至少一个自传递的R质粒。对于产生CAZ-1 / TEM-5或CAZ-5 / SHV-4β-内酰胺酶的肺炎克雷伯菌，其变异程度不同，固化和交配实验表明，可自我编码的R质粒编码TEM-1，CTX-1 / TEM-3，CAZ-2 / TEM-8，CAZ-6 / TEM-24或CAZ-7 / TEM-16β-内酰胺酶不参与肺炎克雷伯菌菌株与肠上皮细胞的粘附。然而，在肺炎克雷伯菌临床分离株中，存在多种抗生素耐药性的关联，包括对广谱头孢菌素的耐药性和粘附特性。

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