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首页> 外文期刊>Journal of Clinical Microbiology >Pulsed-Field Gel Electrophoresis Used To Investigate Genetic Diversity of Haemophilus influenzae Type b Isolates in Australia Shows Differences between Aboriginal and Non-Aboriginal Isolates
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Pulsed-Field Gel Electrophoresis Used To Investigate Genetic Diversity of Haemophilus influenzae Type b Isolates in Australia Shows Differences between Aboriginal and Non-Aboriginal Isolates

机译：用于调查澳大利亚b型流感嗜血杆菌分离株的遗传多样性的脉冲场凝胶电泳显示原住民和非原住民之间的差异

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We used pulsed-field gel electrophoresis to study the epidemiology and population structure of Haemophilus influenzae type b. DNAs from 187 isolates recovered between 1985 and 1993 from Aboriginal children (n = 76), non-Aboriginal children (n = 106), and non-Aboriginal adults (n = 5) in urban and rural regions across Australia were digested with the SmaI restriction endonuclease. Patterns of 13 to 17 well-resolved fragments (size range, ～8 to 500 kb) defining 67 restriction fragment length polymorphism (RFLP) types were found. Two types predominated. One type (n = 37) accounted for 35 (46%) of the isolates from Aboriginals and 2 (2%) of the isolates from non-Aboriginals, and the other type (n = 41) accounted for 2 (3%) of the isolates from Aboriginals and 39 (35%) of the isolates from non-Aboriginals. Clustering revealed seven groups at a genetic distance of ～50% similarity in a tree-like dendrogram. They included two highly divergent groups representing 50 (66%) isolates from Aboriginals and 6 (5%) isolates from non-Aboriginals and another genetically distinct group representing 7 (9%) isolates from Aboriginals and 81 (73%) isolates from non-Aboriginals. The results showed a heterogeneous clonal population structure, with the isolates of two types accounting for 42% of the sample. There was no association between RFLP type and the diagnosis of meningitis or epiglottitis, age, sex, date of collection, or geographic location, but there was a strong association between the origin of isolates from Aboriginal children and RFLP type F2a and the origin of isolates from non-Aboriginal children and RFLP type A8b. The methodology discriminated well among the isolates (D = 0.91) and will be useful for the monitoring of postvaccine isolates of H. influenzae type b.
机译：我们使用脉冲场凝胶电泳来研究 b型流感嗜血杆菌的流行病学和种群结构。从1985年至1993年之间从原住民儿童（ n = 76），非原住民儿童（ n = 106）和非原住民成年人（ n = 5）用 Sma I限制性核酸内切酶消化了澳大利亚城市和农村地区。发现了13到17个分辨率良好的片段（大小范围约为8到500 kb）的模式，这些片段定义了67个限制性片段长度多态性（RFLP）类型。两种类型占主导地位。一种类型（ n = 37）占原住民分离株的35（46％）和非土著人分离株的2（2％），另一种类型（ n < / em> = 41）占原住民分离株的2（3％）和非原住民分离株的39（35％）。聚类显示在树状树状图中，遗传距离相似性在〜50％的七个组。他们包括两个高度分歧的群体，分别代表来自原住民的50个（66％）分离株和非土著人的6个（5％）分离株，另一个遗传上不同的群体代表了来自土著的7个（9％）分离株和81个（73％）的非原住民分离株原住民。结果显示异种克隆种群结构，其中两种类型的分离物占样品的42％。 RFLP类型与脑膜炎或会厌炎的诊断，年龄，性别，采集日期或地理位置之间没有关联，但是原住民儿童的分离株与RFLP F2a型分离株的起源与分离株之间有很强的关联来自非原住民儿童和RFLP A8b型。该方法在分离株之间有很好的区别（ D = 0.91），将对监测 H的疫苗后分离株很有用。 b型流感。

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