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首页> 外文期刊>Journal of Clinical Microbiology >Serodiagnosis of Recently Acquired Toxoplasma gondii Infection with a Recombinant Antigen
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Serodiagnosis of Recently Acquired Toxoplasma gondii Infection with a Recombinant Antigen

机译：用重组抗原对刚获得的弓形虫感染的血清学诊断

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A portion of a cDNA encoding a 35-kDa antigen from Toxoplasma gondii was cloned into the CKS expression vector and expressed inEscherichia coli. By using the enzyme-linked immunosorbent assay (ELISA), the recombinant protein (rP35 antigen) was examined for reactivity with immunoglobulin G (IgG) antibodies in the sera of pregnant women. Of these women, 41 had a toxoplasma serologic profile suggestive of recently acquired T. gondii infection (Sabin-Feldman dye test [DT] titers from 1:256 to 1:32,000, positive IgM ELISA titers from 2.3 to 9.7, positive IgA ELISA from 1 to >28, and acute patterns in the differential agglutination [AC/HS] test) (group I), and 50 women had a toxoplasma serologic profile suggestive of infection acquired in the distant past (low DT titers from 1:16 to 1:512, negative IgM ELISA titers from 0 to 0.8, and chronic patterns in the AC/HS test) (group II). The classification of acute or chronic profile was based on the individual's clinical history as well as the combination of the results of the toxoplasma serological profile. An additional group (group III) was composed of sera from 50 women who were seronegative for T. gondii antibodies in the DT. The results revealed that whereas 85.3% of women in group I had IgG antibodies that reacted with the rP35 antigen, only 8% of women in group II had IgG antibodies that reacted with the same antigen. In immunoblots, the rP35 antigen was recognized by IgG antibodies in a pool of sera from individuals with a toxoplasma serologic profile compatible with acute infection but not in a pool of sera from individuals with a serologic profile characteristic of a chronic infection. These results reveal that IgG antibodies against the P35 antigen are produced during the acute stage of the infection but are uncommon in the latent or chronic phase of the infection. Thus, the rP35 antigen may be a useful serologic marker to differentiate between recently acquired infection and that acquired in the more distant past.
机译：将来自弓形虫的编码35 kDa抗原的cDNA的一部分克隆到CKS表达载体中，并在大肠杆菌中表达。通过酶联免疫吸附试验（ELISA），检查了重组蛋白（rP35抗原）与孕妇血清中免疫球蛋白G（IgG）抗体的反应性。在这些妇女中，有41名具有弓形虫血清学特征，表明最近获得了 T。弓形虫感染（Sabin-Feldman染色法[DT]滴度从1：256至1：32,000，IgM ELISA阳性滴度从2.3至9.7，IgA ELISA阳性滴度从1至> 28，以及差异凝集的急性模式[AC / HS]测试）（第I组），有50名女性的弓形虫血清学特征提示在远古时期已获得感染（DT滴度从1:16到1：512低，IgM ELISA滴度从0到0.8呈负值，和AC / HS测试中的慢性模式）（第二组）。急性或慢性特征的分类基于个体的临床病史以及弓形虫血清学特征结果的组合。另一组（第三组）由50名血清 T血清阴性的女性组成。 DT中的gondii 抗体。结果显示，尽管第一组中85.3％的女性具有与rP35抗原反应的IgG抗体，但是第二组中只有8％的女性具有与相同抗原反应的IgG抗体。在免疫印迹中，rP35抗原在具有与急性感染相容的弓形虫血清学特征的个体的血清中被IgG抗体识别，但在具有慢性感染特征的血清学特征的个体的血清中未被IgG抗体识别。这些结果表明，在感染的急性期产生抗P35抗原的IgG抗体，但是在潜伏期或慢性期很少见。因此，rP35抗原可能是有用的血清标志物，以区分最近获得的感染和更远的过去获得的感染。

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