...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the polymerase chain reaction.
【24h】

Detection of Borrelia burgdorferi infection in Ixodes dammini ticks with the polymerase chain reaction.

机译：用聚合酶链反应检测达克米氏tick中的伯氏疏螺旋体感染。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The polymerase chain reaction (PCR) was used to amplify DNA sequences of the etiologic agent of Lyme disease, Borrelia burgdorferi, and was applied to the detection of the spirochete in its tick vector. The target for PCR amplification was the OSP-A gene of strain B31; analysis of isolates from different geographical areas indicated that this gene could be used to identify most North American isolates. These methods were extended to the analysis of colony-derived and field-collected Ixodes dammini. OSP-A-specific sequences were identified in 15 of 15 colony-derived nymphal ticks that had fed previously on an infected animal; no such amplification products were detected in 8 control ticks. Segregated midgut tissues of field-collected adult and nymphal ticks from Nantucket Island, Mass., and the Crane Reserve, Ipswich, Mass., were examined by both direct fluorescent-antibody (DFA) staining and PCR. The DFA technique identified 16 infected ticks of 30 paired specimens; 15 of these specimens were positive by PCR. One specimen was positive by PCR that was DFA negative. Both live whole ticks and desiccated dead specimens were suitable for this analysis. Because only five ticks are suitable for DFA analysis, the use of PCR may extend the range of specimens that can be analyzed for the presence of the Lyme spirochete.
机译：聚合酶链反应（PCR）用于扩增莱姆病伯氏疏螺旋体的病原体的DNA序列，并用于检测其滴答载体中的螺旋体。 PCR扩增的靶标是菌株B31的OSP-A基因。对来自不同地理区域的分离株的分析表明，该基因可用于鉴定大多数北美分离株。这些方法扩展到了来自菌落的和田间采集的达克斯迷你虫的分析。在先前以感染动物为食的15个菌落来源的若虫tick中，有15个鉴定出OSP-A特异性序列。在8个对照滴答中未检测到此类扩增产物。通过直接荧光抗体（DFA）染色和PCR检测了来自马萨诸塞州楠塔基特岛和马萨诸塞州伊普斯维奇的Crane Reserve的田间采集的成年adult和若虫tick的中肠组织。 DFA技术可识别30个配对标本中的16个受感染的tick。通过PCR这些样品中的15个是阳性的。通过PCR，一个样品是阳性的，而DFA是阴性的。完整的活壁虱和干燥的死亡标本均适合此分析。因为只有五个滴答适合进行DFA分析，所以PCR的使用可能会扩展可以分析莱姆螺旋体存在的标本范围。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号