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首页> 外文期刊>Journal of Clinical Microbiology >Restriction endonuclease analysis of Eikenella corrodens.
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Restriction endonuclease analysis of Eikenella corrodens.

机译：锈蚀艾肯氏菌的限制性核酸内切酶分析。

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Eikenella corrodens is a gram-negative facultative bacillus commonly found in the oral cavity. Although the role of E. corrodens in periodonititis is not clear, its isolation from extraoral infections attests to its pathogenic potential. Previous studies suggested that this species is phenotypically diverse. In the present study, we used restriction endonuclease analysis (REA) to assess the genetic diversity of this species and to explore the applicability of REA in studying the transmission of E. corrodens. Two groups of E. corrodens isolates were used in this study. Group 1 included 47 epidemiologically independent isolates recovered from dental plaques in periodontally healthy subjects and periodontitis patients and from extraoral infections in different geographic areas. Group 2 E. corrodens included 40 isolates recovered from two periodontitis patients and two periodontally healthy subjects. The results indicated that E. corrodens is genetically heterogeneous, as determined by REA. The majority of the group 1 E. corrodens isolates exhibited strain-specific restriction patterns. Forty restriction patterns were distinguishable among the 47 isolates. Analyses of group 2 isolates revealed that three of four subjects harbored more than one clonal type of E. corrodens. In one instance, a periodontitis patient was found to be colonized by six different clones. Furthermore, two different clonal types of E. corrodens were recovered from a single periodontal pocket in this patient. The results indicated that REA may be a useful tool in the epidemiologic investigation of E. corrodens infections.
机译：Eikenella corrodens是常见于口腔中的革兰氏阴性兼性芽孢杆菌。尽管大肠杆菌在牙周炎中的作用尚不清楚，但其与口腔外感染的隔离证明了其致病性。先前的研究表明，该物种在表型上是多样的。在本研究中，我们使用限制性核酸内切酶分析（REA）评估了该物种的遗传多样性，并探讨了REA在研究大肠杆菌的传播中的适用性。在这项研究中使用了两组大肠杆菌（E. corrodens）分离株。第一组包括从牙周健康受试者和牙周炎患者的牙菌斑以及不同地理区域的口腔外感染中回收的47种流行病学独立菌株。第2组大肠杆菌包括从两名牙周炎患者和两名牙周健康受试者中回收的40个分离株。结果表明，通过REA测定，大肠杆菌具有遗传异质性。第1组腐蚀杆菌的大多数分离株表现出菌株特异性的限制性模式。在47个分离株中有40种限制性图谱可区分。对第2组分离株的分析表明，四名受试者中的三名具有一种以上的克隆型大肠杆菌。在一个实例中，发现牙周炎患者被六个不同的克隆定殖。此外，从该患者的单个牙周袋中回收了两种不同类型的大肠杆菌。结果表明，REA可能是对腐蚀的大肠杆菌进行流行病学调查的有用工具。

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