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首页> 外文期刊>Journal of Clinical Microbiology >Agreement between visual and automated UniScept API readings.
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Agreement between visual and automated UniScept API readings.

机译:可视和自动UniScept API读数之间的协议。

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The UniScept API system was evaluated for agreement of visual versus automated readings of both its identification panels and its antimicrobial susceptibility panels. The biochemical responses of 340 oxidase-negative and oxidase-positive fermentative bacterial cultures were read both visually and automatically in the UniScept API 20E system. Automated and visual readings agreed with 99.3% of the biochemicals. Of the 45 tests that disagreed, the tests for indole and citrate were most often in disagreement. A total of 470 fermentative and nonfermentative cultures were used in the UniScept MIC system to compare visual and automated readings of susceptibility results with 17 antimicrobial agents. Agreement within +/- 1 dilution occurred with 94.1% of the enteric fermenters and with 91.7% of the other cultures. Comparison of visual and automated readings resulted in very major discrepancies in 0.95% of the readings, with the largest percentage of discrepancies associated with glucose nonfermenters (1.8%). It was felt that an automated reading is an acceptable alternative to a visual reading of the biochemicals but that 0.95% was just within the acceptable range of the 1% allowable very major discrepancies in the automated reading of susceptibilities.
机译:对UniScept API系统的鉴定板和抗菌药敏板的视觉读数与自动读数的一致性进行了评估。在UniScept API 20E系统中通过视觉和自动读取340种氧化酶阴性和氧化酶阳性发酵细菌培养物的生化响应。自动化和可视化读数与99.3%的生化试剂一致。在不同意的45项测试中,吲哚和柠檬酸盐的测试最不一致。 UniScept MIC系统中总共使用了470种发酵性和非发酵性培养物,以比较17种抗菌剂的药敏结果的可视化和自动化读数。 +/- 1稀释度内的一致性发生在94.1%的肠发酵罐和91.7%的其他培养物中。视觉读数和自动读数的比较导致0.95%的读数存在非常大的差异,其中与葡萄糖非发酵剂相关的差异百分比最大(1.8%)。有人认为,自动读数是生化试剂目测读数的一种可接受的替代方法,但是0.95%只是在敏感性自动读数中1%允许的非常重大差异的可接受范围之内。

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