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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of pseudorabies virus antibody responses in young swine after infection and vaccination by using an immunoglobulin M antibody capture enzyme-linked immunosorbent assay.
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Characterization of pseudorabies virus antibody responses in young swine after infection and vaccination by using an immunoglobulin M antibody capture enzyme-linked immunosorbent assay.

机译：通过使用免疫球蛋白M抗体捕获酶联免疫吸附测定法表征感染和疫苗接种后的年轻猪中的伪狂犬病病毒抗体应答。

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摘要

An immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (MACELISA) was developed for the detection of pseudorabies virus (PRV)-specific IgM antibody in swine sera because false-positive reactions frequently occurred when sera from older swine were tested with an indirect IgM enzyme-linked immunosorbent assay. Monoclonal mouse anti-swine IgM was used as the capturing antibody, and rabbit anti-PRV hyperimmune gamma globulin was used as the indicating antibody. Sera from non-PRV-infected, experimentally infected, vaccinated and challenged, passively immune and challenged, and naturally infected swine were evaluated. The PRV MACELISA had a specificity of 95% and was as sensitive and reproducible as previously reported in direct assays. An antibody response was still detectable with the MACELISA 21 days after inoculation. The PRV MACELISA did not detect a consistent antibody response in sera from swine vaccinated with either killed-PRV or modified live-virus vaccines but did detect an antibody response in sera from passively immune pigs after challenge with virulent PRV. These results indicated that the PRV MACELISA may be useful for the rapid serodiagnosis of recent PRV infection in swine.
机译：开发了一种免疫球蛋白M（IgM）抗体捕获酶联免疫吸附测定（MACELISA）技术来检测猪血清中的伪狂犬病病毒（PRV）特异性IgM抗体，因为当用猪血清测试老年猪血清时，经常发生假阳性反应间接IgM酶联免疫吸附测定。单克隆小鼠抗猪IgM用作捕获抗体，兔抗PRV超免疫丙种球蛋白用作指示抗体。评估了来自未感染PRV，实验感染，接种疫苗和攻击，被动免疫和攻击以及自然感染的猪的血清。 PRV MACELISA的特异性为95％，灵敏度和可重复性与直接测定中以前报道的一样。接种21天后仍可通过MACELISA检测到抗体反应。 PRV MACELISA不能在用灭活PRV疫苗或改良的活病毒疫苗接种的猪的血清中检测到一致的抗体反应，但是在用强毒PRV攻击后却在被动免疫猪的血清中检测到抗体反应。这些结果表明，PRV MACELISA可用于猪近期PRV感染的快速血清诊断。

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