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首页> 外文期刊>Journal of Clinical Microbiology >Application of Directigen FLU-A for the detection of influenza A virus in human and nonhuman specimens.
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Application of Directigen FLU-A for the detection of influenza A virus in human and nonhuman specimens.

机译：Directigen FLU-A在人类和非人类标本中检测甲型流感病毒的应用。

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Directigen FLU-A, a new enzyme immunoassay membrane test, rapidly detects influenza A virus antigen in specimens from patients. Nasopharyngeal washes and pharyngeal gargles were used to determine the effectiveness of the assay as applied to different types of routinely collected clinical samples. All specimens had been previously shown to contain influenza A virus by virus isolation in tissue culture. Directigen FLU-A was 90% sensitive (95% confidence interval, 56 to 99.7%) with nasopharyngeal washes but only 39% sensitive (95% confidence interval, 17 to 64%) with pharyngeal gargles (P = 0.018) when used with samples containing similar amounts of infectious virus (50% tissue culture infective dose, 1.0 to 4.5). The intensity of the positive reaction with Directigen FLU-A did not correlate with the amount of virus in the specimens. Directigen FLU-A was found to detect cell-associated antigen more readily than free virus; only 20 infected cells were required to identify cell-associated influenza A virus antigen, whereas the limit of detection for free virus was 1.63 x 10(3) infectious virus particles. These findings suggest that Directigen FLU-A detects the cell-associated antigen present in clinical specimens rather than free virus. In addition, Directigen FLU-A detected avian and swine influenza A viruses in both cloacal swabs (75% sensitivity) and swine lung homogenates (86% sensitivity), indicating its potential usefulness in the surveillance of nonhuman influenza A viruses.
机译：Directigen FLU-A是一种新的酶免疫测定膜测试，可快速检测患者标本中的A型流感病毒抗原。鼻咽清洗液和咽含漱液用于确定该测定方法的有效性，该方法适用于不同类型的常规收集的临床样品。先前已通过组织培养中的病毒分离显示了所有标本都含有甲型流感病毒。与样品一起使用时，Directigen FLU-A对鼻咽洗液的敏感度为90％（95％置信区间为56％至99.7％），而对于咽部含漱液，仅39％敏感度（95％置信区间为17％至64％）（P = 0.018）含有相似量的传染性病毒（50％组织培养物感染剂量，1.0至4.5）。 Directigen FLU-A的阳性反应强度与标本中的病毒量无关。发现Directigen FLU-A比游离病毒更容易检测细胞相关抗原。仅需20个受感染细胞即可鉴定与细胞相关的A型流感病毒抗原，而游离病毒的检出限为1.63 x 10（3）感染性病毒颗粒。这些发现表明Directigen FLU-A可检测临床标本中存在的细胞相关抗原，而不是游离病毒。此外，Directigen FLU-A在泄殖腔拭子（灵敏度为75％）和猪肺匀浆（灵敏度为86％）中均检测到禽流感和猪A型流感病毒，表明其在监视非人类甲型流感病毒中的潜在用途。

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