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首页> 外文期刊>Journal of Clinical Microbiology >Genotyping of Canadian hepatitis C virus isolates by PCR.
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Genotyping of Canadian hepatitis C virus isolates by PCR.

机译:通过PCR对加拿大丙型肝炎病毒分离株进行基因分型。

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We used PCR for hepatitis C virus (HCV) genotyping with type-specific primers from the core and NS5 genes. Type I was predominant in the general population (58% in blood donors) as well as in different risk groups, such as intravenous drug abusers (58%), blood transfusion recipients (64%), hemophiliacs (62%), and patients with HCV chronic liver disease (76%). Types II, III, and IV were less prevalent in Canada, being found in 10.92, 6.72, and 5.88% of the population, respectively. The type II core primer was not type specific and reacted with the majority of our type I HCV samples, suggesting a false-positive dual infection with two different genotypes (I and II). Digestion of these amplified type I and type II products with restriction endonuclease AccI proved to be very useful in the exclusion of false-positive dual type I and type II infections.
机译:我们使用了来自核心和NS5基因的类型特异性引物进行的丙型肝炎病毒(HCV)基因分型的PCR。 I型在普通人群(献血者中占58%)以及不同风险组中占主导地位,例如静脉吸毒者(58%),输血接受者(64%),血友病(62%)和HCV慢性肝病(76%)。 II型,III型和IV型在加拿大的流行率较低,分别在10.92%,6.72%和5.88%的人口中发现。 II型核心引物不是特定类型的,并且与我们的大多数I型HCV样品反应,表明存在两种不同基因型(I和II)的假阳性双重感染。事实证明,用限制性核酸内切酶AccI消化这些扩增的I型和II型产品对于排除假阳性I型和II型双重双重感染非常有用。

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