Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes.

B I Rosen; A V Parwani; S Lopez; J Flores; L J Saif

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Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes.

机译：通过使用对猪VP4以及人和猪VP7基因具有特异性的核酸探针，在腹泻猪田间样品中轮状病毒的血清型分化。

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Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-derived gene 9 and 4 probes, respectively. Only 17 samples were identified with either homologous VP4 (P)- or VP7 (G)-coding genes or both. One rotavirus identified as G4 and P7 was similar to the previously characterized interserotype rotavirus, SB-1A. Additional hybridization analyses were performed with PCR-derived probes prepared from gene 9 cDNA of the human rotaviruses Wa (G1), DS-1 (G2), and P (G3) and of the porcine rotavirus YM (G11). Eleven of 52 samples collected and analyzed from swine in Ohio, California, and Nebraska were identified as G11. No samples with G1-, G2-, or G3-type specificities were detected among the 25 of 57 rotavirus-positive samples analyzed with human rotavirus-derived probes. Further investigations with a PCR-derived gene 4 probe prepared from porcine rotavirus YM revealed hybridization specificities similar to those of the OSU gene 4 probe.

机译：在美国和加拿大从哺乳或断奶的腹泻猪收集的216份粪便和肠样品中，有57份经RNA电泳和银染鉴定为A型轮状病毒阳性。通过分别与Gottfried和OSU PCR衍生的基因9和4探针杂交，分析了57个和52个轮状病毒阳性样品。仅鉴定了17个具有同源VP4（P）或VP7（G）编码基因或两者的样品。一种被标识为G4和P7的轮状病毒类似于先前鉴定的血清型轮状病毒SB-1A。使用由人轮状病毒Wa（G1），DS-1（G2）和P（G3）以及猪轮状病毒YM（G11）的基因9 cDNA制备的PCR衍生探针进行其他杂交分析。从俄亥俄州，加利福尼亚州和内布拉斯加州的猪收集并分析的52个样品中，有11个被标识为G11。在使用人类轮状病毒衍生的探针分析的57个轮状病毒阳性样本中，有25个没有检测到具有G1，G2或G3型特异性的样本。用猪轮状病毒YM制备的PCR衍生的基因4探针进行的进一步研究表明，杂交特异性与OSU基因4探针相似。

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《Journal of Clinical Microbiology》 |1994年第2期|共7页
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B I Rosen; A V Parwani; S Lopez; J Flores; L J Saif;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses [J] . Martella V, Ciarlet M, Baselga R, Virology . 2005,第1期

机译：VP7和VP4基因的序列分析确定了猪轮状病毒的新VP7基因等位基因，与人类G2轮状病毒具有共同的进化起源
2. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. [J] . B I Rosen, A V Parwani, M Gorziglia, Journal of Clinical Microbiology . 1992,第10期

机译：表征猪轮状病毒血清型的全长和聚合酶链反应衍生的部分长度的Gottfried和OSU基因4探针。
3. Characterization of full-length and polymerase chain reaction-derived partial-length Gottfried and OSU gene 4 probes for serotypic differentiation of porcine rotaviruses. [J] . B I Rosen, A V Parwani, M Gorziglia, Journal of Clinical Microbiology . 1992,第10期

机译：表征猪轮状病毒血清型的全长和聚合酶链反应衍生的部分长度的Gottfried和OSU基因4探针。
4. COMPARISON OF TWO QUANTITATIVE PCR TECHNIQUES FOR PORCINE CIRCOVIRUS TYPE 2(PCV2) NUCLEIC ACID IN FIELD SAMPLES [C] . CK Hiulsager, L Grau-Roma, M Sibila, Congress ofthe International Pig Veterinary Society . 2008

机译：两种定量PCR技术对野生磷毒病型2（PCV2）核酸中的两种定量PCR技术
5. Development and application of full-length and polymerase chain reaction-derived partial-length VP4- and VP7-specific nucleic acid probes for the differentiation of porcine rotavirus serotypes. [D] . Rosen, Blair Ira. 1993

机译：全长和聚合酶链反应衍生的部分长度VP4-和VP7特异性核酸探针在猪轮状病毒血清型分化中的开发和应用。
6. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. [O] . B I Rosen, A V Parwani, S Lopez, 1994

机译：通过使用对猪VP4以及人和猪VP7基因具有特异性的核酸探针在腹泻猪田间样品中轮状病毒的血清型分化。
7. Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes [O] . B I Rosen, A V Parwani, S Lopez, 1994

机译：利用猪酸探针对猪VP4和人和猪VP7基因的核酸探针，腹腔猪的野外样品中血管血清毒性分化

Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes.

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