Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.

M Saito; K Sekiguchi; R Yajima; M Hina; R C Doss; H Kanno

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Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.

机译：使用合成肽制备的单克隆抗体对甲氧西林抗性葡萄球菌的青霉素结合蛋白2'进行免疫学检测。

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Two synthetic peptides 31 and 32 amino acids in length were prepared as deduced from a known amino acid sequence of penicillin-binding protein 2' (PBP2') of methicillin-resistant Staphylococcus aureus. Two monoclonal antibodies were generated from fused cells of myeloma cells and splenic cells of mice immunized with the synthetic peptides. Western blot (immunoblot) analysis demonstrated specific binding of the antibodies to PBP2' of a methicillin-resistant S. aureus strain. An immunoradiometric assay was developed by using these antibodies for simple detection of PBP2'.

机译：如从耐甲氧西林的金黄色葡萄球菌的青霉素结合蛋白2′（PBP2′）的已知氨基酸序列推导，制备了两个长度为31和32个氨基酸的合成肽。从用合成肽免疫的小鼠的骨髓瘤细胞和脾细胞的融合细胞中产生两种单克隆抗体。 Western印迹（免疫印迹）分析表明抗体与耐甲氧西林的金黄色葡萄球菌菌株的PBP2'特异性结合。通过使用这些抗体来简单检测PBP2'，开发了一种免疫放射测定法。

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《Journal of Clinical Microbiology》 |1995年第9期|共3页
作者
M Saito; K Sekiguchi; R Yajima; M Hina; R C Doss; H Kanno;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Discrimination of methicillin-resistant Staphylococcus aureus from methicillin-susceptible Staphylococcus aureus or coagulase-negative staphylococci by detection of penicillin-binding protein 2 and penicillin-binding protein 2' using a bioluminescent enzyme immunoassay [J] . ShigaK., GomiK., NishimuraM., Journal of Immunological Methods . 2013,第1a2期

机译：通过使用生物发光酶免疫分析法检测青霉素结合蛋白2和青霉素结合蛋白2'来区分耐甲氧西林的金黄色葡萄球菌与耐甲氧西林的金黄色葡萄球菌或凝固酶阴性葡萄球菌
2. DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) WITH ANTIBODIES AGAINST SYNTHETIC PEPTIDES DERIVED FROM PENICILLIN-BINDING PROTEIN 2' [J] . Sekiguchi K, Saito M, Yajima R Microbiology and Immunology . 1995,第8期

机译：用抗青霉素结合蛋白2'合成肽段的抗体检测耐甲氧西林金黄色葡萄球菌（MRSA）
3. Production and characterisation of monoclonal and polyclonal antibodies to different regions of the cystic fibrosis transmembrane conductance regulator (CFTR): detection of immunologically related proteins. [J] . Walker J, Watson J, Holmes C, Journal of Cell Science . 1995,第Pta6期

机译：针对囊性纤维化跨膜电导调节剂（CFTR）不同区域的单克隆和多克隆抗体的生产和表征：检测免疫相关蛋白。
4. CHARACTERIZATION OF THE EPITOPE FOR ANTI HUMAN RESPIRATORY SYNCYTIAL VIRUS F PROTEIN MONOCLONAL ANTIBODY 101F USING SYNTHETIC PEPTIDES AND RECOMBINANT F PROTEIN MUTANTS [C] . Marian Kruszynski, Sheng Jiun Wu, Albert Schmidt the European Peptide Symposium . 2007

机译：用合成肽和重组F蛋白突变体表征抗人呼吸道合胞病毒F蛋白单克隆抗体101f的表位
5. The antibiotic resistance of penicillin-binding protein 2A of methicillin-resistant Staphylococcus aureus. [D] . Fuda, Cosimo Carmelo-Stephen. 2007

机译：耐甲氧西林金黄色葡萄球菌青霉素结合蛋白2A的抗生素耐药性。
6. Immunological detection of penicillin-binding protein 2 of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides. [O] . M Saito, K Sekiguchi, R Yajima, 1995

机译：通过使用由合成肽制备的单克隆抗体对耐甲氧西林葡萄球菌的青霉素结合蛋白2进行免疫学检测。
7. Guanine nucleotide binding regulatory proteins and adenylate cyclase in livers of streptozotocin- and BB/Wor-diabetic rats. Immunodetection of Gs and Gi with antisera prepared against synthetic peptides. [O] . Lynch, C J, Blackmore, P F, Johnson, E H, 1989

机译：链脲佐菌素和BB / Wor糖尿病大鼠肝脏中的鸟嘌呤核苷酸结合调节蛋白和腺苷酸环化酶。用针对合成肽的抗血清对Gs和Gi进行免疫检测。
8. Monoclonal Antibodies of Three Different Immunoglobulin G Isotypes Produced byImmunization with a Synthetic Peptide or Native Protein Protect Mice against Challenge with Plasmodium Yoelii Sporozoites. (Reannouncement with New Availability Information) [R] . Mucide, A. K., Bower, J. H., Hoffman, S. L., 1993

机译：通过用合成肽或天然蛋白质免疫小鼠产生的三种不同免疫球蛋白G同种型的单克隆抗体保护小鼠免受恶性疟原虫子孢子的攻击。（重新公布新的可用性信息）

Immunological detection of penicillin-binding protein 2' of methicillin-resistant staphylococci by using monoclonal antibodies prepared from synthetic peptides.

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