Expression and self-assembly of recombinant capsid protein from the antigenically distinct Hawaii human calicivirus.

K Y Green; A Z Kapikian; J Valdesuso; S Sosnovtsev; J J Treanor; J F Lew

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Expression and self-assembly of recombinant capsid protein from the antigenically distinct Hawaii human calicivirus.

机译：来自抗原不同的夏威夷人杯状病毒的重组衣壳蛋白的表达和自组装。

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The Norwalk and Hawaii viruses are antigenically distinct members of the family Caliciviridae and are considered to be important etiologic agents of epidemic gastroenteritis, with most studies focusing on the role of Norwalk virus. To further investigate the importance of Hawaii virus, Hawaii virus-like particles (VLPs) were produced by expression of its capsid protein in the baculovirus system and these VLPs were used as the antigen in an enzyme-linked immunosorbent assay that was efficient in the detection of a serologic response to Hawaii virus. The ready availability of Hawaii VLPs should enable larger-scale epidemiological studies to further elucidate the importance of this agent.

机译：诺沃克和夏威夷病毒是杯状病毒科的抗原性不同成员，被认为是流行性胃肠炎的重要病原体，大多数研究都集中在诺沃克病毒的作用上。为了进一步研究夏威夷病毒的重要性，通过在杆状病毒系统中表达其衣壳蛋白生产了夏威夷病毒样颗粒（VLP），并将这些VLP用作酶联免疫吸附测定法中的抗原，可有效检测对夏威夷病毒的血清学反应夏威夷VLP的现成可用性应使大规模的流行病学研究能够进一步阐明该病原的重要性。

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《Journal of Clinical Microbiology》 |1997年第7期|共6页
作者
K Y Green; A Z Kapikian; J Valdesuso; S Sosnovtsev; J J Treanor; J F Lew;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Self-assembly of in vitro-translated human papillomavirus type 16 L1 capsid protein into virus-like particles and antigenic reactivity of the protein. [J] . S Iyengar, K V Shah, K L Kotloff, Clinical and diagnostic laboratory immunology . 1996,第6期

机译：体外翻译的人乳头瘤病毒16型L1衣壳蛋白的自组装成病毒样颗粒和该蛋白的抗原反应性。
2. Expression of recombinant capsid proteins of chitta virus, a genogroup II Norwalk virus, and development of an ELISA to detect the viral antigen. [J] . Kobayashi S, Sakae K, Suzuki Y, Microbiology and Immunology . 2000,第8期

机译：Chitta病毒（一种基因组II Norwalk病毒）的重组衣壳蛋白的表达，以及用于检测病毒抗原的ELISA的开发。
3. Self-Assembly of the Recombinant Capsid Protein of a Swine Norovirus into Virus-Like Particles and Evaluation of Monoclonal Antibodies Cross-Reactive with a Human Strain from Genogroup II [J] . Horacio Almanza, Carolina Cubillos, Iván Angulo, Journal of Clinical Microbiology . 2008,第12期

机译：猪诺如病毒重组衣壳蛋白自组装成病毒样颗粒并评估与人类基因组II交叉反应的单克隆抗体
4. Expression in Insect Cells and Antigenic Analysis of Recombinant Nucleocapsid Protein of Canine Distemper Virus [C] . Bi Zhenwei, Wang Yongshan, Ouyang Wei, 16th International congress on animal hygiene : animal hygiene, health and welfare as corner stones of sustainable animal production . 2013

机译：犬瘟热病毒在昆虫细胞中的表达及重组核衣壳蛋白的抗原分析
5. Identification and characterization of a heparin-binding region on the L1 major capsid protein of recombinant human papillomavirus virus-like particles and its role in interactions of VLPs with cells. [D] . Joyce, Joseph Gerard. 1998

机译：重组人乳头瘤病毒病毒样颗粒的L1主要衣壳蛋白上肝素结合区的鉴定和表征及其在VLP与细胞相互作用中的作用。
6. Expression and self-assembly of recombinant capsid protein from the antigenically distinct Hawaii human calicivirus. [O] . K Y Green, A Z Kapikian, J Valdesuso, 1997

机译：来自抗原不同的夏威夷人杯状病毒的重组衣壳蛋白的表达和自组装。
7. Self-assembly of human papillomavirus type 1 capsids by expression of the L1 protein alone or by coexpression of the L1 and L2 capsid proteins [O] . M E Hagensee, N Yaegashi, D A Galloway 1993

机译：单独的L1蛋白或通过L1和L2衣壳蛋白的表达，通过表达L1和L2衣壳蛋白的表达，人乳头瘤病毒的自我组装
8. Synergic Investigation Of The Self-Assembly Structure And Mechanism Of Retroviral Capsid Proteins By Solid State NMR, Transmission Electron Microscopy And Multiscale simulation. [R] . Chen, B. 2017

机译：逆转录病毒衣壳蛋白的自组装结构和机制的协同研究，固态核磁共振，透射电子显微镜和多尺度模拟。

Expression and self-assembly of recombinant capsid protein from the antigenically distinct Hawaii human calicivirus.

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