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首页> 外文期刊>Journal of Clinical Microbiology >Reduction of Carriage of Enterohemorrhagic Escherichia coli O157:H7 in Cattle by Inoculation with Probiotic Bacteria
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Reduction of Carriage of Enterohemorrhagic Escherichia coli O157:H7 in Cattle by Inoculation with Probiotic Bacteria

机译：通过接种益生菌减少牛中肠出血性大肠杆菌O157：H7的携带

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Bacteria inhibitory to Escherichia coli O157:H7 were isolated from cattle and evaluated for their potential for reducing carriage of E. coli O157:H7 in calves. Eighteen of 1,200 bacterial isolates from cattle feces and intestinal tissue samples were screened and determined to inhibit the growth of E. coliO157:H7 in vitro. Seventeen of the isolates were E. coli and one was Proteus mirabilis. None produced Shiga toxin. Genomic DNA fingerprinting by pulsed-field gel electrophoresis revealed 13 distinguishable profiles among the 18 isolates. Two calves inoculated perorally with a mixture of all 18 isolates (1010 CFU) appeared to be normal and did not develop signs of clinical disease throughout a 25- to 27-day observation period. These bacteria colonized segments of the gastrointestinal tract and were in feces at the termination of the experiment (25 and 27 days postinoculation) at levels of 50 to 200 CFU/g. Fifteen cannulated calves were studied to determine the efficiency of the probiotic bacteria in reducing or eliminating the carriage of E. coli O157:H7. Nine calves served as controls, with each animal receiving perorally 1010 CFU ofE. coli O157:H7. E. coliO157:H7 was detected intermittently in the rumen samples from all control animals throughout 3 weeks postinoculation, whereasE. coli O157:H7 was shed at various levels in feces continuously throughout the experiment (mean, 28 days).E. coli O157:H7 was isolated from the rumens and colons of eight of nine and nine of nine calves, respectively, at the termination of the study. Six calves each received perorally 1010 CFU of probiotic bacteria and then 2 days later received 1010 CFU of E. coli O157:H7.E. coli O157:H7 was detected in the rumen for only 9 days postinoculation in two animals, for 16 days in one animal, for 17 days in two animals, and for 29 days in one animal. E. coli O157:H7 was detected in feces for only 11 days postinoculation in one animal, for 15 days in one animal, for 17 days in one animal, for 18 days in one animal, for 19 days in one animal, and for 29 days in one animal. At the end of the experiment (mean, 30 days), E. coli O157:H7 was not recovered from the rumen of any of the six animals treated with probiotic bacteria; however, E. coli O157:H7 was recovered from the feces of one of the animals. This animal was fasted twice postinoculation. These studies indicate that selected probiotic bacteria administered to cattle prior to exposure to E. coli O157:H7 can reduce the level of carriage ofE. coli O157:H7 in most animals.
机译：从牛中分离出对大肠杆菌 O157：H7具有抑制作用的细菌，并评估其减少 E携带的潜力。小牛中的O157：H7大肠杆菌。从牛粪和肠组织样品中分离出的1200种细菌分离物中的18种被筛选出并确定抑制 E的生长。大肠杆菌O157：H7体外。其中有17株是E。大肠杆菌，其中之一是 Proteus mirabilis 。没有一个产生志贺毒素。通过脉冲场凝胶电泳对基因组DNA进行指纹分析，揭示了18个分离株中的13个可区分的谱。在全部25至27天的观察期内，用全部18种分离株（10 10 CFU）的混合物经口接种的两只小牛似乎正常，没有出现临床疾病的迹象。这些细菌定居在胃肠道的各个部分，并在实验结束时（接种后25天和27天）以50至200 CFU / g的水平排泄在粪便中。研究了十五只空心小牛，以确定益生菌减少或消除Eem携带的效率。大肠杆菌O157：H7。 9只犊牛作为对照，每只动物经口接受 E的10 10 CFU。大肠杆菌O157：H7。 E。在接种后的3周内，从所有对照动物的瘤胃样品中均间歇性地检测到O157：H7，而E则是Eem。在整个实验过程中（平均28天），大肠杆菌O157：H7在粪便中以不同水平连续脱落。在研究结束时，分别从九只犊牛中的八只和九只犊牛中的九只的瘤胃和结肠中分离出了O157：H7大肠杆菌。 6只犊牛经口口服益生菌10 10 CFU，然后两天后接受 E 10 10 CFU。大肠杆菌 O157：H7。 E。在两只动物中，瘤胃中仅在接种后9天检测到O157：H7，在一只动物中检测到16天，在两只动物中检测到17天，在一只动物中检测到29天。 E。一只动物的粪便中仅检测到大肠杆菌O157：H7，接种后仅11天，一只动物检测15天，一只动物检测17天，一只动物检测18天，一只动物检测19天，在一只动物中放置29天。在实验结束时（平均30天）， E。从用益生菌处理过的六只动物的瘤胃中未检出大肠杆菌O157：H7。但是， E。从其中一只动物的粪便中回收了大肠杆菌O157：H7。该动物在接种后禁食两次。这些研究表明，选择的益生菌细菌在暴露于 E之前已施用于牛。大肠杆菌O157：H7可以降低 E的运输水平。大多数动物中的O157：H7大肠杆菌。

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