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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Salmonella spp. in clinical specimens by capture enzyme-linked immunosorbent assay.
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Detection of Salmonella spp. in clinical specimens by capture enzyme-linked immunosorbent assay.

机译：沙门氏菌的检测。在临床标本中通过捕获酶联免疫吸附测定。

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A capture enzyme-linked immunosorbent assay (ELISA; Kirkegaard and Perry Laboratories, Gaithersburg, Md.) was used to detect Salmonella spp. in clinical and artificially inoculated specimens. In patients with bacteremia caused by Salmonella spp., 48% (12 of 25) and 82% (13 of 16) of serum and urine specimens, respectively, were positive for Salmonella spp., as determined by ELISA. All serum and urine specimens collected from healthy individuals (25 specimens) or patients whose blood cultures grew gram-negative bacteria other than Salmonella spp. (18 specimens) were negative for Salmonella spp., as determined by ELISA. For blood culture bottles in which Salmonella spp. (16 specimens) was grown the ELISA was positive (100%), while it was negative for all the 65 blood culture bottles in which gram-negative bacteria other than Salmonella spp. (42 specimens) or gram-positive bacteria (23 specimens) were grown. All samples of urine (16 specimens), stool (8 specimens), serum (16 specimens), culture media (12 specimens), and blood culture bottles (reported sterile after 2 weeks of incubation; 16 specimens) that were artificially inoculated with 10(3) to 10(7) CFU of four species of Salmonella per ml were positive by ELISA. Similar specimens inoculated with or containing various species other than Salmonella were negative by this test. Thus, ELISA offers a promising opportunity for the rapid detection of Salmonella spp. in clinical microbiology laboratories.
机译：用捕获酶联免疫吸附测定法（ELISA; Kirkegaard and Perry Laboratories，Gaithersburg，MD）检测沙门氏菌。在临床和人工接种的标本中。通过ELISA检测，沙门氏菌引起的菌血症患者血清和尿液样本分别为48％（25分之12）和82％（16分之13）。从健康个体（25个样本）或血液培养中生长出除沙门氏菌属以外的革兰氏阴性菌的患者的所有血清和尿液样本。 （18个标本）沙门氏菌属阴性，通过ELISA测定。用于沙门氏菌属的血培养瓶。 （16个标本）已生长ELISA呈阳性（100％），而对于所有65个除沙门氏菌属以外的革兰氏阴性菌的血培养瓶，ELISA均为阴性。 （42个样本）或革兰氏阳性细菌（23个样本）得以生长。人工接种了10份尿液的所有尿液样本（16个样本），粪便（8个样本），血清（16个样本），培养基（12个样本）和血培养瓶（孵育2周后报告无菌; 16个样本）。 ELISA检测每毫升四种沙门氏菌的（3）至10（7）CFU呈阳性。通过该测试，接种或含有沙门氏菌以外的其他物种的相似标本为阴性。因此，ELISA为快速检测沙门氏菌提供了有希望的机会。在临床微生物学实验室。

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