...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea.
【24h】

Detection of genes for fimbrial antigens and enterotoxins associated with Escherichia coli serogroups isolated from pigs with diarrhea.

机译：检测与腹泻猪分离的大肠杆菌血清群相关的纤维抗原和肠毒素基因。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A total of 1,226 Escherichia coli strains isolated from 1979 to 1989 from pigs with diarrhea were examined for serogroup and fimbrial antigen F4 (K88) production. Four main patterns of isolation of the various serogroups were observed, depending on the ages of the pigs from which isolates were obtained and the production of F4. In pattern I, serogroups O8:K"S16", O9:K35, O9/O101:K30, O9/O101:K103, O9 (group), O20:K101, and O64:K"V142" were predominant in pigs aged 0 to 6 days (41.9% of isolates) and were less frequent in pigs aged 7 to 27 days (24.6% of isolates) but were rarely found in pigs aged 28 to 60 days (4.0% of isolates). In pattern II, the F4-associated serogroups O8:K"4627", O157:K"V17", O149:K91, and O147:K89 were predominant in pigs aged 7 to 27 days (29.8% of isolates) and in pigs aged 28 to 60 days (35.0% of isolates). In pattern III, serogroups O8 (group), O115:K"V165", and O147:K89 were rarely isolated from pigs aged 0 to 6 days but were equally distributed in pigs aged 7 to 27 days (10.1% of isolates) and in pigs aged 28 to 60 days (10.9% of isolates). In pattern IV, serogroups O138:K81, O139:K82, O141:K85ac, O45:K"E65", and O26:K60 were most frequently isolated in pigs aged 28 to 60 days (19.3% isolates). Over the period from 1979 to 1989, the proportion of isolates belonging to serogroups of pattern II and the proportion of F4 isolates within the serogroup O157:K"V17" declined, whereas the proportion of isolates of serogroups O147:K89, O8:K"S16", and O9:K35 increased. For 228 isolates selected from the most important serogroups, good agreement was observed between the results of gene probes and immunofluorescence for the detection of fimbrial antigens F4 (K88), F5 (K99), F6 (987P), and F41 and between the results of gene probes and biological assays for the detection of heat-labile enterotoxin (LT) and heat-stable enterotoxins a and b (STa and STb). The STa gene was mostly associated with isolates of pattern I serogroups, which had the F5, F6, and F41 genes alone or in various combinations. The LT and/or STb genes, with the F4 gene, mostly were observed in isolates of pattern II serogroups. The STb gene alone was observed mostly in isolates of pattern III serogroups, although isolates were negative for all fimbrial antigen genes. Similarly, isolates of pattern IV serogroups were negative for all fimbrial antigen genes and rarely positive for the enterotoxin genes. However, verotoxin production was associated with isolates of serogroups O138:K81 and O139:K82. The most important pathotypes among enterotoxigenic isolates in this study were F4:LT:STb, F5:STa, STb, F5:F41:STa, F4:STb, F6, STa, and LT.
机译：从1979年至1989年共从腹泻猪中分离出1,226株大肠杆菌，检测其血清群和纤维抗原F4（K88）的产生。观察到四种不同血清群的主要分离模式，这取决于获得分离株的猪的年龄和F4的产生。在模式I中，血清组O8：K“ S16”，O9：K35，O9 / O101：K30，O9 / O101：K103，O9（组），O20：K101和O64：K“ V142”占主导地位6至6天（分离株的41.9％），在7至27天的猪（分离株的24.6％）中发病率较低，但在28至60天的猪（分离株的4.0％）中很少见。在模式II中，与F4相关的血清群O8：K“ 4627”，O157：K“ V17”，O149：K91和O147：K89的血清群在7至27天的猪（分离株的29.8％）和年龄大的猪中占主导地位。 28至60天（占分离株的35.0％）。在模式III中，很少从0至6天龄的猪中分离出血清型O8（组），O115：K“ V165”和O147：K89，但平均分布于7至27天的猪中（10.1％的分离株）和猪龄为28至60天（分离株的10.9％）。在模式IV中，在28至60天龄的猪中最常分离出血清群O138：K81，O139：K82，O141：K85ac，O45：K“ E65”和O26：K60（分离株为19.3％）。在1979年至1989年期间，属于模式II血清群的分离株比例和O157：K“ V17”血清群中F4分离株的比例下降，而针对O147：K89，O8：K“血清群的分离株的比例下降S16“和O9：K35增加。对于从最重要的血清群中选择的228个分离株，在检测纤维抗原F4（K88），F5（K99），F6（987P）和F41的基因探针结果和免疫荧光结果之间以及在基因探针和生物学测定法，用于检测不耐热肠毒素（LT）和热稳定肠毒素a和b（STa和STb）。 STa基因主要与模式I血清群的分离株相关，后者单独或以各种组合形式具有F5，F6和F41基因。 LT和/或STb基因，以及F4基因，大多在II型血清型分离株中观察到。尽管分离株对所有纤维抗原基因均呈阴性，但仅在模式III血清群的分离株中仅观察到STb基因。同样，IV型血清群的分离株对所有纤维抗原基因均为阴性，而对肠毒素基因则很少为阳性。但是，维毒素的产生与血清群O138：K81和O139：K82的分离株有关。在这项研究中，产肠毒素菌株中最重要的病理型是F4：LT：STb，F5：STa，STb，F5：F41：STa，F4：STb，F6，STa和LT。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号