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首页> 外文期刊>Journal of Clinical Microbiology >High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii.
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High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii.

机译：Sabin-Feldman染料测试与酶联免疫吸附测定法之间的抗体滴度高度相关，该酶联免疫吸附测定法检测了弓形虫核苷三磷酸水解酶的免疫球蛋白G抗体。

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摘要

An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.
机译：建立了一种酶联免疫吸附测定法，以检测针对弓形虫特异性和显性抗原的核苷三磷酸水解酶的免疫球蛋白G抗体，并将该测试的灵敏度和特异性与Sabin-Feldman染料测试的灵敏度和特异性进行了比较。在对37例阳性和50例阴性人类血清进行的比较研究中，比较研究观察到100％的一致性。酶联免疫吸附试验中的抗体滴度表示为最高阳性血清稀释度的倒数，仅为染色试验中相对于阳性血清的81％（37中的30）的100倍。

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